ELECTRONIC POSTER - ismrm

States; 5 Chemistry & Chemical Engineering, Shenzhen University, Shenzhen, Guangdong, China; 6 Department
of Pharmacology, Oxford University, Oxford, United Kingdom; 7 Department of Physiology, University of
Auckland, Auckland, New Zealand
End stage diabetes is often associated with heart failure, which is the leading cause of death. We are the first to investigate the heart
function in STZ-induced diabetic rats longitudinally with and without triethylenetetramine (TETA) treatment using High Field MRI.
Gradient echo cine method was used to determine cardiac function. We found that the cardiac ejection fraction decreased with
prolonged diabetic status and oral TETA treatment improves ejection fraction in diabetic rats. The results suggest that TETA treatment
is beneficial to diabetic heart failure and warrant further clinical investigation.
14:00 3641. Manganese-Enhanced MRI Combined with Delayed Enhancement MRI Detects
Injured Border Zone Myocardium in a Pig Ischemia-Reperfusion Model
Rajesh Dash 1 , Jaehoon Chung 1 , Yuka Matsuura 1 , Fumiaki Ikeno 1 , Jennifer Lyons 1 ,
Tomohiko Teramoto 1 , Alan C.Y. Yeung 1 , Michael V. McConnell 1,2 , Todd J. Brinton 1 ,
Phillip Harnish 3 , Phillip C. Yang 1
1 Division of Cardiovascular Medicine, Stanford University Medical Center, Stanford, CA, United States;
2 Department of Electrical Engineering, Stanford University , Stanford, CA, United States; 3 Eagle Vision
Pharmaceutical Corporation, Exton, PA, United States
Manganese-enhanced MRI (MEMRI) detects Mn 2+ uptake into viable cells, a distinct mechanism from gadolinium delayed
enhancement MRI (DE-MRI). We tested whether combined DE-MRI plus MEMRI would delineate peri-infarct border zone injury in
a pig ischemia-reperfusion (IR). Pigs were imaged by cardiac MRI 3 weeks post-IR. 3D DE-MRI scar volume correlated with
histopathologic scar volume, but MEMRI scar volume was significantly smaller than DE-MRI scar volume. The border zones of DE-
MRI scar, which were also positive by MEMRI, showed decreased SNR compared to remote zone MEMRI SNR. Combined MEMRI
and DE-MRI may identify injured border zone myocardium in ischemic cardiomyopathy.
14:30 3642. Diffusion Tensor Shape Measurements of Infarcted Myocardium in Porcine Models
Using Three Phase Geometric Analysis
Yin Wu 1,2 , Ed Xuekui Wu 2,3
1 Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Shenzhen,
Guangdong, China; 2 Laboratory of Biomedical Imaging and Signal Processing, The University of Hong Kong,
Pokfulam, Hong Kong; 3 Dept. of Electrical and Electronic Engineering, The University of Hong Kong,
Pokfulam, Hong Kong
Previous DTI studies on infarcted LV myocardium structure usually investigated diffusivity and diffusion anisotropy. In current study,
diffusion tensor shape with a combination of linear, planar and spherical measures are examined and illustrated on a three-phase space
in porcine models. Results show that the measurements of tensor shape have significant alteration in infarcted myocardium and are
more sensitive to detect subtle change of diffusion properties than conventionally used parameters. Infarct location shows no apparent
influence on myocardium structural degradation. This study gives insights into myocardium structural alteration and demonstrates
potential application of DTI in detecting infarcted heart remodeling.
15:00 3643. Whole Mount Heart Histology: A New Gold Standard for Myocardial Damage
Validation in Experimental Cardiac MRI Studies?
Yuesong Yang 1 , Kela Liu 1 , Dan Wang 1 , Mihaela Pop 1 , Jay Detsky 1 , Yingli Lu 1 , Alexander
J. Dick 1 , Martin J. Yaffe 1 , Graham A. Wright 1
1 Imaging Research, Sunnybrook Health Sciences Centre, University Of Toronto, Toronto, Ontario, Canada
Triphenyltetrazolium chloride (TTC) stain is commonly used for the validation of myocardial damage in experimental cardiac MRI
studies using various animal species such as mice, dogs and pigs. However, subtle myocardial damage, border zone or infarct
heterogeneity associated with myocardial infarction (MI) is difficult to recognize on TTC stains. In this study, we investigated the
feasibility of whole-mount heart histology that preserved the 3D morphology with a digital display at the microscopic level as a new
alternative in the validation of myocardial damage in a porcine model of MI in experimental cardiac late-enhancement (LE) MRI
studies.
Thursday 13:30-15:30 Computer 33
13:30 3644. Comparison of Scar Morphology by 3D Multi-Contrast Late Enhancement MRI, 3D
DW-MRI and Histology in a Pig Model of Chronic Infarct
Mihaela Pop 1 , Venkat Ramanan, Yuesong Yang, Nilesh Ghugre, Beiping Qiang, Elliot R.
McVeigh 2 , Alexander J. Dick 3 , Graham A. Wright
1 Medical Biophysics, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada;
2 Biomedical Engineering, Johns Hopkins University, United States; 3 Cardiology, Sunnybrook Research Institute
Accurate assessment of the scar extent and tissue remodeling during healing is very important. To better understand the scar
morphology associated with chronic infarct in a porcine model, we have developed and tested (ex vivo) a 3D pulse sequence based on
multi-contrast late enhancement (MCLE) and a non-contrast 3D diffusion-weighted DW sequence, and compare the results against
histopathology. We have found that 3D MCLE identifies fine heterogeneity of scar, and compares well the classification of pathology
to that from apparent diffusion coeficient maps using DW-MRI.