ELECTRONIC POSTER - ismrm

14:30 4519. Metabolite Abnormalities in Rhesus Monkeys During Withdrawal of
Methamphetamine: A 1 H MR Spectroscopy Study at 3T
Shaolin Yang 1 , Svetlana Chefer 1 , D. Bruce Vaupel 1 , Xi Chen 1 , Pradeep K. Kurup 1 ,
Thomas J. Ross 1 , Elliot A. Stein 1 , Yihong Yang 1
1 National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, United States
A proton magnetic resonance spectroscopy ( 1 H-MRS) study was performed to examine the metabolite abnormalities in the Rhesus
monkeys during the abstinence from a long history of methamphetamine (METH) self-administration. The 1 H-MRS spectral data were
acquired from the frontal lobe and striatum of Rhesus monkeys at 3T in 5 separate sessions (1-day, 1-week, 1-month, 3-month, and 6-
month abstinence, respectively). Compared to the control group (n=5), the total choline (tCho) level in the striatum is significantly
elevated during the early abstinence (up to 1 month) and continued to be elevated after 3 months of drug withdrawal in the METHabstinence
group (n=10).
15:00 4520. Brain Metabolites B1-Corrected Proton T1 Mapping in the Rhesus Macaque at 3T
Songtao Liu 1 , Roman Fleysher 2 , Lazar Fleysher 2 , Chan-Gyu Joo 3 , Eva-Maria Ratai 3 , R
Gilberto Gonzalez 3 , Oded Gonen 2
1 National Institutes of Health Clinical Center, Bethesda, MD, United States; 2 New York University Medical
Center, New York, NY, United States; 3 Massachusetts General Hospital, Boston, MA, United States
The rhesus macaque brain is an advanced model system for the study of neurological diseases. To correct for unknown T1 weighting
in MRS quantification, the B1 corrected T1s of NAA, Cho and Cr in gray and white matter structures of rhesus macaques were
measured at 3T. Data was acquired with 3D multivoxel proton MRSI at 180uL resolution. The macaques’ NAA, Cr and Cho T1s are,
1232, 1238 and 1107ms. These values are in agreement with human 3T in vivo results.
15:30 4521. High Spatiotemporal Resolution for Molecular Imaging with BIRDS
Daniel Coman 1,2 , Robin A. de Graaf 3 , Douglas L. Rothman, 23 , Fahmeed Hyder, 23
1 Diagnostic Radiology, Yale University, New Haven, CT, United States; 2 Quantitative Neuroscience with
Magnetic Resonance (QNMR), Yale University, New Haven, CT, United States; 3 Diagnostic Radiology and
Biomedical Engineering, Yale University, New Haven, CT, United States
Biosensor Imaging of Redundant Deviation in Shifts (BIRDS) is used for pH/temperature mapping in rat brain. The BIRDS method
relies on strong dependence of non-exchangeable protons from thulium based macrocyclics, e.g., TmDOTP 5- and TmDOTMA - , for
temperature and pH. Although high speed 2D CSI allows ~10μL voxels in rat’s cerebral cortex within 5 minutes, many applications
require whole brain coverage and high spatiotemporal resolution. Here we demonstrate a 3D CSI of a 2.5×2.5×2.5 cm field-of-view
with 1μL voxels in 5 minutes using reduced k-space spherical encoding, which represents an order of magnitude sensitivity
enhancement from the 2D CSI data acquisition.
Tuesday 13:30-15:30 Computer 89
13:30 4522. Investigation of Spatial Distribution of Metabolites in Rat Brain at Elevated Plasma
Glucose Levels
Vladimir Mlynarik 1 , Cristina Cudalbu 1 , Hanne Frenkel 1 , Nicolas Costers 1,2 , Rolf
Gruetter 1,3
1 Laboratory of Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, Lausanne,
Switzerland; 2 Katholieke Universiteit Leuven, Leuven, Belgium; 3 Departments of Radiology, Universities of
Lausanne and Geneva, Switzerland
Absolute metabolic maps of 12 metabolites were obtained in rat brain at different plasma glucose (Glc) concentrations (6.4 – 19
mmol/L) using short-echo-time proton spectroscopic imaging. A satisfactory linear fit of brain Glc concentration versus plasma Glc
concentration in cortex, hippocampus and thalamus was found. The slope of this fit was the same within experimental error in all
measured brain structures. The linear fit of increased lactate concentrations at increased plasma Glc levels was poor, indicating that
other factors such as stress or impaired metabolism during long-term anesthesia can affect the lactate concentration in brain.
Concentrations of myo-inositol, taurine, ascorbate, phosphoethanolamine, glutamine, GABA, N-acetylaspartate, total creatine, total
choline and macromolecules were not substantially affected by acute hyperglycemia.
14:00 4523. The Effect of Electroconvulsive Shock on the Neurochemical Profile in the Live Rat:
Neurogenesis & Glutamate
S. David Smith 1 , June-Hee Park 2 , Michael Ma 3 , Mei Yu 4 , Shaonan Zhang 3 , Mirjana
Maletic-Savatic 5 , Annemie Van der Linden 6 , G. Enikolopov 2 , Helene Benveniste 4
1 Medical, Brookhaven National Laboratory, Upton, NY, United States; 2 Cold Spring Harbor Laboratory, Cold
Spring Harbor, NY, United States; 3 Stony Brook University, United States; 4 Anesthesiology, Stony Brook
University, Stony Brook, NY, United States; 5 Baylor College of Medicine, TX, United States; 6 University of
Antwerpen, Antwerpen, Belgium
We tested the hypotheses that 1) the electroconvulsive shock (ECS) induced increase in the 1.28ppm neural stem cell biomarker could
be tracked using LCModel software and 2) that ECS being an effective anti-depressant treatment would result in changes in glutamate
detectable by LCModel software. Analysis of our 1HMRS data acquired from the rat dentate gyrus before and after ECS demonstrated
that the 1.28ppm signal was too low to be tracked by LCModel software (CRLB