ELECTRONIC POSTER - ismrm

14:30 4496. Effect of Mesenchymal Stem Cells on the Cerebral Microvascularisation in a Rat
Model of Stroke: MRI Study
Anaick Moisan 1 , Emmanuelle Grillon 1 , Emmanuel L. Barbier 1 , Florence De Fraipont 2 ,
Chantal Remy 1 , Marie-Jeanne Richard 2,3 , Olivier Detante 1,4
1 INSERM U836 / Joseph Fourier University (Functional and Metabolic Imaging), Grenoble Institute of
Neurosciences (GIN), Grenoble, France, Metropolitan; 2 INSERM U823 / Joseph Fourier University, Albert
Bonniot Institute, Grenoble, France, Metropolitan; 3 Cell and Tissue Therapy Unit, University Hospital,
Grenoble, France, Metropolitan; 4 Stroke Unit, Department of Neurology, University Hospital, Grenoble,
France, Metropolitan
In a rat model of stroke, the effects of human mesenchymal stem cells (hMSC) on the evolution of microvascularisation were studied.
Seven days after transient cerebral ischemia, rats received a 10µL intracerebral administration of either cell culture medium or 4×105
hMSC. Two groups of healthy control rats underwent the same treatment. Groups were followed by MRI during 21 days (ADC,
cerebral blood volume (CBV), vessel size (VSI)). One day after IC administration, hMSC abolish the CBV increase commonly
observed after transient cerebral ischemia. VSI estimates suggest that hMSC also delay the vasodilation secondary to cerebral
ischemia.
15:00 4497. Microvascular Density Quantitative Changes After Rat Embolic Stroke Using MRI
Asamoah Bosomtwi 1,2 , Quan Jiang 2,3 , Guanlian Ding 2 , Li Zhang 2 , Zheng Gang Zhang 2,3 ,
Mei Lu 4 , James R. Ewing 2,3 , Michael Chopp 2,3
1 Yerkes Imaging Center, Emory University, Atlanta, GA, United States; 2 Neurology, Henry Ford Hospital,
Detroit, MI, United States; 3 Physics, Oakland University, Rochester, MI, United States; 4 Biostatistics and
Research Epidemiology, Henry Ford Hospital, Detroit, MI, United States
We investigated vascular remodeling after stroke using MRI microvascular density (MVD) measurement and gold standard immunohistochemistry
staining. We demonstrate that MRI MVD detect the microvascular status of brain tissue with different ischemic
damage. The MVD measured by MRI was highly correlated with histological measures of MVD. Our data demonstrate that MRI
MVD measurement can quantitatively evaluate microvascular remodeling after stroke.
Thursday 13:30-15:30 Computer 87
13:30 4498. Stem Cell Treatment of Hemorrhagic Lesions Investigated by Longitudinal DTI
Study of a Monkey Model
Chenlu Feng 1 , Qinyu Zeng 1 , Zhengguang Chen 2 , Tie-Qiang Li 3 , Xiaoming Yin 1 , Jianwei
Huo 1 , Ming Feng 4 , Renzhi Wang 4
1 Department of Radiology, Beijing Coal General Hospital, Beijing, China; 2 Department of Radiology, First
Hospital of Tsinghua University, Beijing, China; 3 Department of Medical Physics, Karolinska University
Hospital, Stockholm, Sweden; 4 Department of Neurosurgery, Peking Union Medical College Hospital, Peking
Union Medical College, Beijing, China
The treatment of hemorrhagic lesion with neuronal stem cell procedure was studied by diffusion tensor imaging in a monkey model
using a clinical 3T MRI system. The longitudinal changes of the fractional anisotropy indicate that the procedure at the dosage of 0.5-
2.5 million cells is very effective and DTI is a useful tool to monitor the time course of the neuronal repair and fiber track regeneration
process.
14:00 4499. Cerebral Blood Volume Imaging of Spreading Depression in S218L Mouse Model of
Familial Hemiplegic Migraine Type 1
Kwangyeol Baek 1,2 , Katherina Eikermann_Haerter 3 , Woo Shim 1,2 , Cenk Ayata 3 ,
Guangping Dai 1 , Jeong Kon Kim 4 , Bruce R. Rosen 1 , Jaeseung Jeong 2 , Young Ro Kim 1
1 Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, MA, United States; 2 Bio and
Brain Engineering, KAIST, Daejeon, Korea, Republic of; 3 Radiology, Massachusetts General Hospital,
Charlestown, MA, United States; 4 Radiology, Asan Medical Center, Seoul, Korea, Republic of
Spreading depression (SD) is a propagating wave of cellular depolarization, and implicated in pathophysiology of migraine and periinfarct
depolarization. In last decade, MRI started to be used for non-invasive imaging of SD, but not much is known about the
neurovascular coupling in SD, especially for subcortical regions. We investigated CBV changes induced by SD in cortical and
subcortical regions using intravenous contrast agent. We observed marked CBV increase (up to 20%) in cortex, striatum, and
hippocampus, but not in thalamus. The CBV response gradually reached peak ~5 min after the neuronal depolarization, suggesting
neurovascular coupling is largely modified in SD.
14:30 4500. The Quantification of Tissue Sodium Concentration Using 23 Na-Magnetic
Resonance Microscopy at 7 T: Probing the Acute Stroke Phase
Friedrich Wetterling 1,2 , Lindsay Gallagher 3 , Mhairi I. Macrae 4 , Sven Junge 5 , Andrew
John Fagan, 1,6
1 School of Physics, Trinity College Dublin, Dublin, Ireland; 2 Computer Assisted Clinical Medicine, University
of Heidelberg, Mannheim, Germany; 3 Glasgow Experimental MRI Centre, , Division of Clinical Neuroscience,
Faculty of Medicine, University of Glasgow, Glasgow, Scotland, United Kingdom; 4 Glasgow Experimental
MRI Centre, Division of Clinical Neuroscience, Faculty of Medicine, University of Glasgow, Glasgow,