ELECTRONIC POSTER - ismrm

15:00 3243. In Vivo Fiber Tracking of Muscle Anatomy in Rodents (Oryctolagus Cuniculus) on
a Clinical 3T MRI System
Daniel Güllmar 1 , Tobias Siebert 2 , Kay Leichsenring 2 , Carolin Küpper 2 , Reinhard
Blickhan 2 , Jürgen R. Reichenbach 1
1 Medical Physics Group, Department of Diagnostic and Interventional Radiology, Jena University Hospital,
Jena, Germany; 2 Science of Motion, Institute of Sport Science, Friedrich-Schiller-University Jena, Jena,
Germany
This work aims to facilitate diffusion tensor imaging on a clinical 3T MRI system in order to reconstruct the inner shank muscle
architecture of rodents. Using a slightly modified EPI-DTI sequence and 3D anatomic scan, we were able to reconstruct the muscular
structure within feasible scan time (approx. 45 min). The promising results will be validated by examining the myostructure using
high-resolution (0.07 mm) 3D reconstruction techniques (Microscribe MLX) ex-vivo.
Muscle & Miscellaneous
Hall B Monday 14:00-16:00 Computer 6
14:00 3244. Functional Assessment of Skeletal Muscle in Mice Lacking Myostatin by
Multiparametric Functional (Mpf-) NMR in Vivo
Celine Baligand 1,2 , Helene Gilson 3 , Jacques C. Menard 1,2 , Olivier Schakman 3 , Claire Wary 1,2 ,
Jean-Paul Thissen 3 , Pierre Georges Carlier 1,2
1 NMR Laboratory, Institute of Myology, F-75651 Paris, France; 2 CEA, I²BM, MIRCen, IdM NMR Laboratory,
F-75651 Paris, France; 3 Unite de Diabetologie et Nutrition, Universite Catholique de louvain, B-1200 Brussels,
Belgium
Inhibiting myostatin (mstn) causes spectacular increase in muscle mass, and has opened the path to therapeutic approaches. Yet
possible compromised force production have been reported in isolated muscle. We investigated vascular and metabolic response to
exercise in vivo in mstn-/- and wild-type mice using interleaved arterial spin labeling NMR imaging and 31P spectroscopy. Specific
force and maximum perfusion were identical. Mitochondrial oxidative capacities were reduced in mstn-/-, while hyperemia was
prolonged. These integrated results formed coherent evidence of a non-pathologic shift towards a more glycolytic metabolism in this
model as was confirmed by histology.
14:30 3245. Effects of Resistance Trainging and β-Hydroxy-β-Methylbutyrate (HMB) on Muscle
Fiber CSA and Lean Body Mass in Aged Rats: A DTI and DEXA Study
Ihssan S. Masad 1,2 , Y-M Park 3 , S-R Lee 3 , Jacob M. Wilson 3 , Paul C. Henning 3 , Bahram
H. Arjmandi 3 , Samuel Colles Grant 1,2 , J-S Kim 3
1 Department of Chemical & Biomedical Engineering, The Florida State University, Tallahassee, FL, United
States; 2 National High Magnetic field Laboratory, Tallahassee, FL, United States; 3 Department of Nutrition,
Food & Exercise Sciences, The Florida State University, Tallahassee, FL, United States
SSarcopenia, age-related muscle wasting, has drastic medical and financial impacts on the elderly population. In this study, diffusion
tensor imaging (DTI), which is sensitive to muscle architecture, is employed to assess the potential benefits of β-hydroxy-βmethylbutyrate
(HMB) and resistance training (RT) in a pre-clinical model of aged rats. DTI was used to evaluate muscle cross
sectional area (CSA) while dual energy X-ray absorptiometry was utilized to assess lean body mass (LBM). As well as increasing
LBM, results demonstrate that RT caused hypertrophy in aged soleus muscle, as indicated by increased ADC as well as increased
second and third eigenvalues.
15:00 3246. Diffusion Tensor Imaging of the Calf Muscles at 1.5T: Diffusion Property
Differences Between Athletes and Non-Athletes
Yoshikazu Okamoto 1 , Yuka Kujiraoka 2 , Manabu Minami 3
1 Radiology, University of Tsukuba Hospital , Tsukuba, Ibaraki, Japan; 2 Radiology, Tsukuba Memorial Hopital,
Japan; 3 University of Tsukuba hospital, Japan
We compared@FA, eigenvalues, and ADC for bilateral gastrocnemius medialis (GCM),gastrocnemius lateralis (GCL), soleus
(SOL) and anterior tibial (AT) muscles between athletically trained and not-trained skeletal muscle. In all eight muscles, all three
eigenvalues and ADC were lower in trained muscle than in not-trained one. There were significant differences in all muscles as for
ă1 and ADC (P