ELECTRONIC POSTER - ismrm

Wednesday 13:30-15:30 Computer 88
13:30 4510. In Vivo DTI-Derived Axial Diffusivity Correlates with Neurological Assessments in
EAE-Affected Mice
Joong Hee Kim 1 , Anne H. Cross 2 , Sheng-Kwei Song 3
1 Radiology, Washington University , St. Louis, MO, United States; 2 Neurology, Washington University, St.
Louis, MO, United States; 3 Radiology, Washington University, St. Louis, MO, United States
Diffusion tensor imaging (DTI) was used to examine the spinal cords of mice with experimental autoimmune encephalomyelitis
(EAE), an animal model of Multiple Sclerosis. Compared to age-matched controls, EAE-affected mice exhibited a statistically
significant decrease in axial diffusivity in spinal cord white matter. The decrease of axial diffusivity was parallel to disease severity
examined by clinical scoring of EAE mice. The axial diffusivity threshold analysis on EAE-affected mice enabled quantifying the
extent of abnormal or damaged axons, which correlated with four independent neurological assessments.
14:00 4511. Contrasting Roles for CD4 and CD8 T Cells in a Murine Model of T1 Black Hole
Formation
Istvan Pirko 1 , Jeremiah McDole 2 , Yi Chen 2 , Scott R. Dunn 3 , Diana M. Lindquist 3 , Aaron
J. Johnson 2
1 Department of Neurology, Mayo Clinic, Rochester, MN, United States; 2 Department of Neurology, University
of Cincinnati, Cincinnati, OH, United States; 3 Imaging Research Center, Cincinnati Children's Hospital Medical
Center, Cincinnati, OH, United States
TMEV infection of mice is an accepted model of multiple sclerosis. In C57B6/J mice, the formation of T1 black holes (T1BH) is
detectable in this model. In this study we confirmed that CD8 T cells are the main contributors to T1BH formation, whereas CD 4 T
cells prevent T1BH formation. We also determined that the involved CD8 T cells are classic epitope specific cytotoxic T cells. T1BH
formation is thought to represent neuronal/axonal damage in MS; therefore, it is plausible that CD8 T cells play an important effector
role targeted at neurons and axons in MS-related neuroinflammatory diseases.
14:30 4512. Monitoring Demyelination in a Cuprizone Mouse Model with Longitudinal and
Quantitative MRI Measurements
Jonathan D. Thiessen 1 , Yanbo Zhang 2 , Handi Zhang 2 , Lingyan Wang 2 , Richard Buist 3 ,
Jiming Kong 4 , Xin-Min Li 2 , Melanie Martin 1,5
1 Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada; 2 Psychiatry, University of
Manitoba; 3 Radiology, University of Manitoba; 4 Human Anatomy and Cell Science, University of Manitoba;
5 Physics, University of Winnipeg
Magnetic resonance imaging methods capable of quantifying changes due to demyelination can improve both the diagnosis and
understanding of white matter diseases such as multiple sclerosis. T 2 -weighted and magnetization transfer images (MTI) were
acquired weekly in control (n=4) and cuprizone-fed mice (n=4) from 2 to 6 weeks of treatment. Diffusion tensor imaging, quantitative
MTI, high-resolution T 2 -weighted imaging, and histopathology were used to analyze ex vivo tissue. All in vivo methods showed
significant differences longitudinally in the corpus callosum of the cuprizone-fed mouse. All in vivo and ex vivo methods showed
significant differences in the corpus callosum between groups.
15:00 4513. Correlation of Fractional Anisotropy (FA) Changes in Demyelination Lesion with
Its Surrounding Edema in an Experimental Model
Krithika Balasubramanian 1 , Senthil S. Kumaran 1 , Uma Sharma 1 , Naranamangalam R.
Jagannathan 1
1 Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, Delhi, India
Evaluation of sequential changes in fractional anisotropy (FA) in demyelination lesion and associated edema in an experimental rat
model of demyelination was carried out at 4.7T. Results showed that both FA(lesion) and FA(edema) decreased during demyelination
till day 11. Decreased FA(lesion) is attributed to the damage of myelin which progressed till day 11 while reduced FA(edema) is due
to breakdown of blood-brain barrier (BBB). From day 15 remyelination set in along with repair of BBB, which led to increased
FA(lesion) and FA(edema). Our study thus showed that DTI may aid in better understanding of the pathophysiology of de- and remyelination.
Thursday 13:30-15:30 Computer 88
13:30 4514. Study of the Pathophyisology of Demyelination in an Experimental Model:
Correlation of Lesion Volume with T2, Apparent Diffusion Coefficient (ADC) and Fractional
Anisotropy (FA)
Krithika Balasubramanian 1 , Uma Sharma 1 , Senthil S. Kumaran 1 , Naranamangalam R.
Jagannathan 1
1 Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, Delhi, India
Evaluation of variation in T2, ADC and FA with the lesion volume at various stages of de- and re-myelination in a demyelinating rat
model was carried out at 4.7 T. During demyelination lesion size, T2 and ADC increased while FA decreased indicating loss of
myelin, while these values were reversed during remyelination. A strong positive correlation was observed between lesion volume and