ELECTRONIC POSTER - ismrm

significantly in rats exposed to ECS suggesting a role for glutamate as an anti-depressant and/or as an important instigator of synaptic
plasticity.
14:30 4524. In Vivo Studies on a Hyperpolarized Choline Contrast Agent: Design and
Implementation of a New Biomarker
Hyla Allouche-Arnon 1 , Aaron K. Grant 2 , Elena Vinogradov 2 , Xiaoen Wang 3 , Robert E.
Lenkinski 3 , Ayelet Gamliel 1 , Ruppen Nalbandian 1 , Lucio Frydman 4 , John Moshe
Gomori 1 , Claudia Monica Barzilay 5 , Rachel Katz-Brull 1,6
1 Department of Radiology, Hadassah Hebrew University Medical Center, Jerusalem, Israel; 2 Department of
Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston,, MA, United States;
3 Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA,
United States; 4 Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel; 5 Medicinal
Chemistry-School of Pharmacy, Hebrew University of Jerusalem, Jerusalem, Israel; 6 BrainWatch Ltd., Tel-
Aviv, Israel
Choline metabolism is known to be altered in neurodegeneration and malignancy. In order to enable the monitoring of choline
metabolism in a direct and non-invasive manner in vivo, a stable-isotope labeled analog of choline namely, [1,1,2,2-D 4 ,2- 13 C]-choline
chloride, was designed and implemented for hyperpolarized magnetic resonance applications. The position enriched with 13 C in this
molecule presents with both a long T 1 (35 sec) and a chemical shift that differentiates choline from its metabolites. Here we report on
the first in vivo studies of carbon-13 hyperpolarized [1,1,2,2-D 4 ,2- 13 C]-choline that suggest it is a promising new agent for metabolic
imaging by MRI and MRSI.
15:00 4525. Metabolite Profiling of Mild Hypothermia by 1H-MRS
Kannie W. Y. Chan 1,2 , April M. Chow 1,2 , L. Xiao 1,2 , Ed X. Wu 1,2
1 Laboratory of Biomedical Imaging and Signal Processing, The University of Hong Kong, Pokfulam, Hong
Kong SAR, China; 2 Department of Electrical and Electronic Engineering, The University of Hong Kong,
Pokfulam, Hong Kong SAR, China
Hypothermia has a profound effect on the protection of brain. However, its exact mechanisms remain to be elucidated. Hypothermia
induces changes in brain metabolites. 1H-MRS can detect changes in metabolites and hence help us to have a better understanding of
hypothermia. Our data shows changes in several metabolites in the cortex and thalamus. These metabolites are associated with cascade
of events that lead to neuroprotection and thermoregulation, which are similar to ex vivo and microdialysis findings. This real time
and site specific monitor of metabolites at 7T gives insight into how hypothermia protects the brain from various insults.
Wednesday 13:30-15:30 Computer 89
13:30 4526. Effects of Lactoferrin on Altered Brain Metabolism in Pup Rats After Prenatal
Exposure to Dexamethasone
Yohan van de Looij 1,2 , Pierre Larvaron 1 , Emmanuel Somm 1 , Bing Wang 3 , Rolf
Gruetter 2,4 , Stéphane V. Sizonenko 1 , Petra S. Hüppi 1
1 Division of Child Growth & Development, Department of Pediatrics, University of Geneva, Geneva,
Switzerland; 2 Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne,
Lausanne, Switzerland; 3 Department of Nutrition and Health, Nestlé Research Center, Lausanne, Switzerland;
4 Department of Radiology, University of Geneva and Lausanne, Geneva and Lausanne, Switzerland
Rat model of glucocorticoids exposure during gestation has shown reduction in brain weight. At P7, alterations of cerebral metabolism
in the cortex and the hippocampus have been observed by in vivo 1H-MRS. Lactoferrin (Lf) is an iron-binding glycoprotein secreted
in milk which has an antioxidant activity. The aim of this work was to evaluate the neuroprotective effect of Lf following prenatal
exposure to glucocorticoids by high field localized 1H-MRS. Neurochemical profiles of the pup rat brains confirmed the altered brain
development after Dex exposure and revealed the potential protective effect of Lf given to gestational and lactating dams.
14:00 4527. Proton MRS in the Late Stage of Neonatal Hypoxic-Ischemic Cerebral Injury
April M. Chow 1,2 , Iris Y. Zhou 1,2 , Shu Juan Fan 1,2 , Kannie W.Y. Chan 1,2 , Kevin C. Chan 1,2 ,
Ed X. Wu 1,2
1 Laboratory of Biomedical Imaging and Signal Processing, The University of Hong Kong, Pokfulam, Hong
Kong SAR, China; 2 Department of Electrical and Electronic Engineering, The University of Hong Kong,
Pokfulam, Hong Kong SAR, China
Neonatal hypoxic-ischemic (HI) cerebral injury is major cause of permanent damage to neuronal cells. The neonatal brain undergoes
regenerative processes late after HI injury. While 1 H MRS has been employed to investigate metabolic changes during acute-phase of
HI injury, roles of major neurochemicals as markers for neurodegeneration and neuroprotection at late stage are also important for
studying the neurophysiological changes. In this study, we showed that alteration in the metabolism at late stage in cortical and
subcortical structures is associated with neonatal HI cerebral injury. This may provide insights into the plastic changes and adaptive
modifications within brain following injury.