TABLE OF CONTENTS - BGB Analytik AG

Why Filter Your Samples
for HPLC?
• To protect your column
against plugging
(blockage) from sample
particulate matter.
• To protect your injection
valve components from
possible damage,
scratching and increased
wear by sample
particulate matter.
• To minimize downtime.
Why Purchase Agilent
Syringe Filters?
Agilent offers the most
popular sizes, porosities
and membrane types at
attractive pricing. All of our
syringe filters are pre-sterilized
by gamma irradiation,
and our regenerated cellulose
filters are batch-tested under
HPLC conditions.
Sample Filtration
Various methods of sample filtration can be used to clarify samples that need further analysis
or where particulate matter may cause a problem. Agilent provides a range of standard and
economy syringe filters conveniently housed in inert polymeric housings for easy use and
disposal, as well as the innovative Mini-UniPrep Syringeless Filters from Whatman.
Syringe Filters
Membrane syringe filters are used most often to clarify small-volume sample solutions prior to
HPLC and ion chromatography. The membrane filters are contained in an inert polymeric
housing. No glue or binders are used in their construction to ensure that no extractables are
present. The housing is designed to spread the sample solution over the entire surface of the
membrane so that maximum membrane capacity is used. Syringe filters are ready-to-use and
are quite convenient. You merely attach the Luer-tipped syringe filled with the sample solution
to the housing and push the sample through the pre-cleaned filters.
How to Select the Right Membrane Syringe Filter
www.agilent.com/chem/supplies
Syringe Filters
Filter types should be selected based on sample volume. All filter inlets are female
Luer-compatible, have inert polypropylene or polycarbonate housings and come
in three diameters:
• 30 mm filters are designed for larger sample volumes or for solvent filtration and offer
increased filtration speed. The holdup volume is less than 50 µL.
• 25 mm Econofilters offer a moderately wide cross-sectional area (4.2 cm2) with a holdup
volume of less than 50 µL.
• 13 mm filters are ideal for most applications and offer the best compromise between
holdup volume and sample volume. Sample volumes are typically in the 1-10 mL range
and the holdup volume is less than 10 µL.
Porosity should be determined by the size of potential particulates in your sample.
Finer porosities require more pressure to filter.
Two porosities are available: 0.45 micron pores to remove particles that are detrimental to
most columns and 0.20 micron pores to remove the smallest particles.
Sample Preparation
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