TABLE OF CONTENTS - BGB Analytik AG

Column and Mobile Phase Guidelines:
Reversed Phase
www.agilent.com/chem/supplies
Column Selection
HPLC columns consist of two parts, the column chemistry and hardware. For the proper column
chemistry, consult the catalog section for each type of bonded phase. For choosing column
hardware and particle sizes, consult the section on column sizes and rapid separations,
including Agilent ZORBAX Rapid Resolution and Rapid Resolution HT columns, as well as
Solvent Saver and Capillary columns and PrepHT columns.
Type of
Surface
HPLC Column
Stationary Phase Column Dimensions
Pore Size Particle Size Length
Chemical Properties
Chemical Lifetime/Sensitivity
Retention Factor
Pore Size Selection
Inner
Diameter
Physical Properties
Efficiency
Speed
Choose a column packing with small pore (60-120Å) if the solute molecular weight is less
than about 5000. Otherwise, use column packing with the 300Å pore size.
Particle Size Selection
The standard particle size for HPLC columns is 5 µm with 3.5 µm now dominant for method
development. If high-speed analyses or higher resolution analyses are required, packing with
1.8 µm and 3.5 µm particles can be used. Shorter columns with these particles can produce
faster high-resolution separations, with the 1.8 µm particle size in Rapid Resolution HT
columns, providing the highest efficiency. The 3.5 µm particle size operates at a routine
operating pressure and can be used on all LC's. Short (50 mm and shorter) 1.8 µm RRHT
columns can be used on optimized standard LC's, while the longer columns may require a
higher pressure LC (one supporting pressures greater than 400 bar).
LC and LC/MS
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