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LC and LC/MS ZORBAX Rx ZORBAX Rx Analysis of Diazepam on Rx-C18 Column: ZORBAX Rx-C18 880967-302 3.0 x 250 mm, 5 µm Mobile Phase: 35% H2O: 65% MeOH Flow Rate: 0.5 mL/min 622 www.agilent.com/chem/supplies • Rx-C18 is recommended for alternate selectivity at low pH relative to Eclipse XDB-C18 and StableBond SB-C18; for higher temperature applications, StableBond is recommended. This column has a higher carbon load than SB-C18 columns (12% vs. 10%). • Rx-C18 offers high stability and good peak shape for low pH applications. • Rx-C18 is manufactured using dimethyloctadecylsilane, is non-endcapped and provides excellent stability up to pH 8. • Rx-C8 is the same product as SB-C8. Column Specifications Bonded Phase Pore Size An Rx-C18 column is used for this USP analysis of diazepam and the internal standard ethylparaben. The Solvent Saver 3.0 mm i.d. Rx-C18 column reduces solvent usage by 60% over what would be used if the analysis were done on a 4.6 x 250 mm column. Surface Area Temp. Limits 1. Ethylparaben 2. Diazepam pH Range* Endcapped 1 0.0 2.5 Time (min) 5.0 7.5 2 Carbon Load ZORBAX Rx-C18 80Å 180 m2/g 60°C 2.0-8.0 No 12% ZORBAX Rx-C8 80Å 180 m2/g 80°C 1.0-8.0 No 5.5% Specifications represent typical values only. *At pH 6-9 highest column stability for all silica based columns is obtained by operating at temperatures < 40°C and using lower buffer concentrations in the range of 0.01 - 0.02M. LCRX001
ZORBAX Rx Hardware Description Size (mm) Particle Size (µm) www.agilent.com/chem/supplies Rx-C18 USP L1 ZORBAX Rx Rx-C8 USP L7** Semi-Preparative 9.4 x 250 5 880967-202 880967-201 Analytical 4.6 x 250 5 880967-902 880967-901 Analytical 4.6 x 150 5 883967-902 883967-901 Rapid Resolution 4.6 x 150 3.5 863967-902 863953-906 Rapid Resolution 4.6 x 100 3.5 861967-902 861953-906 Rapid Resolution 4.6 x 75 3.5 866967-902 866953-906 Solvent Saver 3.0 x 250 5 880967-302 880975-306 Solvent Saver 3.0 x 150 5 883967-302 883975-306 Solvent Saver Plus 3.0 x 150 3.5 863967-302 863954-306 Solvent Saver Plus 3.0 x 100 3.5 861967-302 861954-306 Narrow Bore 2.1 x 150 5 883700-902 883700-906 Narrow Bore RR* 2.1 x 100 3.5 861767-902 861753-906 Guard Cartridge, 2/pk 9.4 x 15 7 820675-115 820675-115 Guard Cartridge, 4/pk 4.6 x 12.5 5 820950-914 820950-913 Guard Cartridge, 4/pk 2.1 x 12.5 5 821125-915 821125-915 Guard Hardware Kit 9.4 x 15 840140-901 840140-901 Guard Hardware Kit 820888-901 820888-901 PrepHT Cartridge Columns (require endfittings kit 820400-901) PrepHT Cartridge 21.2 x 250 7 877967-102 877250-106 PrepHT Cartridge 21.2 x 150 7 877150-106 PrepHT Cartridge 21.2 x 150 5 870150-906 PrepHT Cartridge 21.2 x 100 5 870100-906 PrepHT Cartridge 21.2 x 50 5 870050-906 PrepHT Guard Cartridge, 2/pk 820212-914 820212-915 Guard Cartridge Hardware 820444-901 820444-901 PrepHT endfittings, 2/pk 820400-901 820400-901 *RR: Rapid Resolution 3.5 µm **Rx-C8 is the same product as SB-C8 LC and LC/MS 623
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TABLE OF CONTENTS Featured Products
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7683B Automatic Liquid Sampler Deli
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Agilent 1200 Series LC Systems and
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1200 Series Purification Systems De
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Agilent 6300 Series Ion Trap LC/MS
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Make the Change to ICP-MS with Agil
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Stratagene Real-time PCR Solutions
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MS Certified Liners www.agilent.com
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Focus on what you do best, and leav
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Japan Yokogawa & Co., Ltd. 7-336, K
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Screw top vials with fixed inserts
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Preassembled vial pack Pre-Assemble
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Magnetic crimp caps for CTC, 5188-5
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Polypropylene Crimp/Snap Top Vials
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On-column syringe Push pull Push bu
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CTC Analytics PAL Autosampler Suppl
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X-type fixed needle, G4200-80118 Re
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Sample tray, G6500-80101 Well plate
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Carrier Gas Purification Gas supply
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HPIB and GPIB Cables Integrators, I
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Sample Preparation Improve the qual
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Move more quickly toward the method
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R 1 Sulfonic acid-modified divinyl
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O Si O Si (CH2) 17CH3 Si O Si(CH 3)
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Structure of ethyl (C2) silane, cov
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Si O Si O Si OH Structure of diol s
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Additional Agilent SampliQ SPE Phas
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20-Port Vacuum Manifold Assembly, 5
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Membrane Econofilters, 5185-5830 Re
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Tips & Tools For more information,
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GC and GC/MS For over 40 years, Agi
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BTO septa, 5183-4757 Summary of Pre
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MS certified split liner, 5188-6576
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Agilent splitless liners Single tap
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Ferrule Selection Recommendations w
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Universal column nut, 5181-8830 Col
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FID cleaning kit, 9301-0985 Flame I
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ECD make up gas adapter ECD packed
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GC/MS Systems • Less downtime for
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MS interface column nut, 05988-2006
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Electron Impact (EI) Ion Source Ion
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Foreline Pump Vacuum Systems and Pu
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Agilent's Über One Test Probe Mixt
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High Efficiency Capillary GC Column
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Structure of Dimethylpolysiloxane D
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CH 3 CH 3 CH 3 O Si Si O Si CH3 CH3
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CH 3 O Si CH 3 Structure of Dipheny
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CH 3 DB-35ms Chromatograms Environm
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Structure of Dimethylpolysiloxane D
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CH 3 O Si O Si Structure of Dipheny
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O CH 3 C 6H 5 Si O Si CH 3 50% C 6H
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O Si Structure of trifluoropropylme
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DB-17, 5 in. cage ID (mm) Length (m
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DB-1701P, 5 in. cage ID (mm) Length
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Agilent LTM System CAM LTM Column M
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DB-1ms LTM Column Modules ID (mm) L
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DB-17ms LTM Column Modules ID (mm)
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HP-PLOT Q LTM Column Modules www.ag
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GC Columns Stationary Phase Applica
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ASTM Methods Method Designation Met
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Baseline Instability or Disturbance
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Loss of Resolution GC Column Troubl
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Organochlorine Pesticides III Colum
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Nitrogen/Phosphorus Containing Pest
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Herbicides II Column: DB-210 122-02
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Environmental Applications, Semivol
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Semivolatile Compounds, EPA Method
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PBDEs by ECD Column: DB-XLB 15 m x
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PCBs by EPA Method 8082 Column: DB-
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Organotin Compounds II Column: HP-5
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EPA Method 8061 (Phthalate Esters)
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Environmental Applications, Volatil
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Extended Analyte List for EPA Metho
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EPA Method 551 Column: DB-1 122-103
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EPA Air Analysis Method TO-15 (1 pp
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Polyunsaturated Fatty Acid Methyl E
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1100/1200 Series LC System Needles
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1200 Rapid Resolution Connection Ca
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Standard flow cell Standard flow ce
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pH and Mobile Phase The choice of m
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Analyte charge Silica surface charg
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Method development at mid pH (4-9)
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Page 599 and 600: Selectivity Changes for Basic Compo
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PLgel Columns Agilent Technologies
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Polystyrene: Calibration Curves for
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Separation of Simple-Sugar and Suga
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High Resolution of 24 Amino Acids U
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Novel Bidentate C18-C18 Bonding for
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LC/MS Analysis of Angiotensin on Ex
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ZORBAX GF-250 and GF-450 Gel Filtra
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ZORBAX Bio-SCX Series II Provides M
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High Sensitivity with Capillary Col
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Proteins in a Complex Sample by 2-D
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HPLC Troubleshooting Symptom Type P
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Analgesics: High-Speed PrepHT Isocr
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Cough Formula Mixture: Fast and Eff
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Use ZORBAX Extend-C18 for Alternate
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Peptides: Effect of TFA Concentrati
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Peptide Map - Electronic Flow Contr
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Peptide RP-HPLC/ESI-MS Using NH4OH
Page 737 and 738:
Catecholamines/Biogenic Amines: Rap
Page 739 and 740:
Proteins: Effect of Bonded Phase Co
Page 741 and 742:
BSA Tryptic Digest on RRHT Column:
Page 743 and 744:
Polymethacrylate: GPC Separation Co
Page 745 and 746:
Triton X-114: Decreasing Run-time b
Page 747 and 748:
Organic Acids Separated on ZORBAX S
Page 749 and 750:
Anilines, Separation of Substituted
Page 751 and 752:
Explosives from Soil Extract Column
Page 753 and 754:
Herbicides: Use of Short, Small Par
Page 755 and 756:
Triazine Pesticides on Bonus-RP and
Page 757 and 758:
Plant Hormones: Rapid Gradient Elut
Page 759 and 760:
Aromatic Flavorings Column: Eclipse
Page 761 and 762:
Carbohydrates in Colas Column: ZORB
Page 763 and 764:
Flavoring Agents Column: ZORBAX SB-
Page 765 and 766:
Parabens: High Speed Separation Col
Page 767 and 768:
Water-Soluble Vitamins Column: ZORB
Page 769 and 770:
Fast Vitamin E Analysis on Rapid Re
Page 771 and 772:
Xanthines: Higher Resolution, Same
Page 773 and 774:
Anesthetics, Local: Bonded Phase Se
Page 775 and 776:
Antihistamines Column: ZORBAX Rx-C8
Page 777 and 778:
Chiral Separation of Fluoxetine Ena
Page 779 and 780:
Chiral Separation of Tolperison Ena
Page 781 and 782:
Metronidazole: Updating USP Methods
Page 783 and 784:
Opiate Drugs, Basic Compounds Showi
Page 785 and 786:
Steroids Column A: Eclipse XDB-Phen
Page 787 and 788:
Tricyclic Antidepressants, Screenin
Page 789 and 790:
Dexamethasone, USP Method: Rapid An
Page 791 and 792:
Sulfonamides - Fast Analysis with R
Page 793 and 794:
CE and CE/MS Achieve the sensitivit
Page 795 and 796:
Tips & Tools Agilent offers a varie
Page 797 and 798:
µPAGE Basic Kits Includes 3 µPAGE
Page 799 and 800:
Extended Light Path (Bubble Cell) B
Page 801 and 802:
www.agilent.com/chem/supplies CE an
Page 803 and 804:
www.agilent.com/chem/supplies CE an
Page 805 and 806:
Alignment Interfaces Capillary cass
Page 807 and 808:
CE/MS Accessories www.agilent.com/c
Page 809 and 810:
CE Standards & Reagents Premade buf
Page 811 and 812:
CE System Start-up and Test Kits Ch
Page 813 and 814:
Accessories www.agilent.com/chem/su
Page 815 and 816:
ICP-MS The power you need for ultra
Page 817 and 818:
Gas Line Supplies www.agilent.com/c
Page 819 and 820:
Peristaltic Pump Tubing and Supplie
Page 821 and 822:
Concentric Nebulizer, quartz Use wi
Page 823 and 824:
Quartz spray chamber, G1820-65337 S
Page 825 and 826:
Interface Cone Guide for Agilent IC
Page 827 and 828:
Electron multiplier, 5184-1983 Ion
Page 829 and 830:
Integrated Autosampler Supplies Des
Page 831 and 832:
ISIS Supplies www.agilent.com/chem/
Page 833 and 834:
Miscellaneous Supplies Description
Page 835 and 836:
Single Element Standards Descriptio
Page 837 and 838:
UV-Vis Keeping your system operatin
Page 839 and 840:
Semi-Micro Cell with PTFE Lid Semi-
Page 841 and 842:
Round Aperture Flow Cell Rectangula
Page 843 and 844:
Deuterium lamp assembly, 2140-0605
Page 845 and 846:
UV-Vis Standards & Reagents Our che
Page 847 and 848:
0905-1175..........................
Page 849 and 850:
123-0131LTM........................
Page 851 and 852:
125-5032E..........................
Page 853 and 854:
19091N-133.........................
Page 855 and 856:
200-0185...........................
Page 857 and 858:
5064-8286..........................
Page 859 and 860:
5183-4428..........................
Page 861 and 862:
5982-4411..........................
Page 863 and 864:
840300-908.........................
Page 865 and 866:
959746-906.........................
Page 867 and 868:
G1530-61580........................
Page 869 and 870:
G3270-80026........................
Page 871 and 872:
1,4-Butanediol ....................
Page 873 and 874:
Dicrotophos .......................
Page 875 and 876:
1,2,3,4,6,7,8-Hepta chloro dibenzof
Page 877 and 878:
Octadecane ........................
Page 879 and 880:
Triethylenetetramine (4 isomers) ..
Page 881 and 882:
Caps 8-425 Screw Top No Septa......
Page 883 and 884:
GC Installation Kits Basic Installa
Page 885 and 886:
Moisture Traps Cont. Silica Gel, Gr
Page 887 and 888:
Vials Cont. 2 mL Glass ............
Page 889 and 890:
Easy Ways To Order* • For Interna
Page 891 and 892:
Agilent Technologies Order Form Agi
Page 893 and 894:
Page 895 and 896:
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