TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Agilent Prep
Superior Loadability on Agilent Prep C18
with Basic Compounds
Column: Agilent Prep-C18
443905-902
4.6 x 150 mm, 5 µm
Mobile Phase: 50% 0.1%TFA:
50% ACN
Flow Rate: 1 mL/min
Sample: 10 µL
Doxepin/
mAU
Amitriptyline
4000
0.5–50 mg/mL 3500
664 www.agilent.com/chem/supplies
3000
2500
2000
1500
1000
Agilent Prep Columns show better resolution and loadability than competitor columns.
500
Steroids: Easy Scalability Using Agilent Prep Columns
Column A: Agilent Prep-C18
443905-902
4.6 x 150 mm, 5 µm
Column B: 443905-102
21.2 x 150 mm, 5 µm
Column C: 413910-302
30 x 150 mm, 10 µm
Column D: 413910-502
50 x 150 mm, 10 µm
Mobile Phase: 55% Water:45% ACN
Flow Rate: 0.7 mL/min
14.87 mL/min
29.77 mL/min
85.37 mL/min
Temperature: Ambient
Detector: 240 nm
Sample: 2 µL
42.4 µL
170 µL
488 µL
Agilent Prep-C18 shows excellent scalability making method transfer
simple and predictable.
0
0
Agilent Prep C18 Competitor W-C18
A
1 2
0 2 4 6 8 10 12
B
1
2
0 2 4 6 8 10 12
C
D
α = 1.6
40
35
30
25
20
15
10
5
0
α = 1.57
1 2 3 4 5 6 7 8 9
0 1 2 3 4 5 6 7 8 9
Time (min) Time (min)
1
0 2 4 6 8 10 12
1
mAU
1. Hydrocortisone
2. Testosterone (in MeOH @ 1mg/mL)
0 2 4 6
Time (min)
8 10 12
2
2
LCPLC01
LCPLC02