TABLE OF CONTENTS - BGB Analytik AG

Protein In-gel Tryptic Digestion Kit
The Protein In-gel Tryptic Digestion Kit contains a complete set of reagents to perform tryptic
digestions on colloidal Coomassie or fluorescent dye-stained protein bands excised from
polyacrylamide gels.
• Process approximately 150 samples
• Easy-to-follow, 7-step protocol produces accurate and reproducible digestions
in suitable form for mass spectrometric analysis
• Designed to function with a wide range of protein band concentrations,
greater than ~20 ng/band
• Includes all buffers and six reagents, including Trypsin, needed for protocol
Protein In-gel Tryptic Digestion Kit
Description Part No.
Protein in-gel tryptic digestion kit 5188-2749
Spin Filters
Spin Filters are polypropylene spin tubes with cellulose acetate filter membranes that can be
used to remove particulate matter from samples such as serum, plasma, or protein samples
diluted in aqueous buffers through low-speed centrifugation of samples through the filter.
• 50 - 500 µL sample volume capacity
• 2 mL sample receiver volume capacity
• Sample holdup volume is < 5 µL
• Contains a cellulose acetate membrane with a 0.22 µm pore size
• Designed to be used with standard benchtop microcentrifuge at maximum 16,000 g
Spin Filters
Description Unit Part No.
0.22 µm cellulose acetate 25/pk 5185-5990
Accessories
Description Part No.
Buffer A, 1 L, for loading, washing, and equilibrating 5185-5987
Buffer B, 1 L, for eluting 5185-5988
PEEK end fitting, 2 µm frit 5185-5995
www.agilent.com/chem/supplies
mRP-C18 High Recovery Protein Columns
LC and LC/MS
717