TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
ZORBAX PrepHT
High Purity and High Recovery with ZORBAX PrepHT Columns
Sample: Antianginal drugs
Mass-based fraction collection using
ZORBAX SB-C18 column shows high purity
and high recovery of each compound
(Application Note publication number 5988-
7113EN). The separation of the three
antianginal drugs was successfully done in
a single run with high recovery and >90%
purity. Separations up to 2000 mg are
possible depending on the complexity of
separation.
658 www.agilent.com/chem/supplies
Abundance (mAU)
8000000
6000000
4000000
2000000
DMSO 1 2 3
DAD
0 1 2 3 4
Time (min)
5 6 7
Vial 2 – 343
Vial 1 – 347
Vial 3 – 389
0 1 2 3 4
Time (min)
5 6 7
Amount
Nifedipin [mg]
1. Nifedipine
2. Nimodine
3. Nisoldipine
Amount
Nifmodipin [mg]
Amount
Nifsoldipin [mg]
Fraction 1 18.90 0.11 0.16 Purity Nifedipin 98.6%
Fraction 2 0.29 17.66 0.77 Purity Nifmodipin 94.4%
Fraction 3 0.49 1.66 18.36 Purity Nifsoldipin 89.5%
Recovery [mg] 19.68 19.43 19.29
Recovery [%] 101.3 102.0 101.9
MSD
LCPHT01