TABLE OF CONTENTS - BGB Analytik AG

StableBond SB-C18 Shows Excellent Stability at Low pH
and High Temperature (pH 0.8, 90°C)
Column: ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
Column: ZORBAX Rx-C18
883967-902
4.6 x 150 mm, 5 µm
Relative Retention
1.0
0.8
0.6
0.4
0.2
0.0
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Mobile Phase: 50% Methanol/50% Water
with 1.0% TFA
Test Solute: Toluene
Temperature: 90°C
0 5,000 10,000 15,000 20,000 25,000 30,000
Column Volumes of Mobile Phase Purge
LCSB001
ZORBAX 80Å StableBond
StableBond SB-C18 (Diisobutyl-C18)
ZORBAX Rx-C18 (Dimethyl-C18)
Competitor A-C18
Competitor B-C18
Competitor C-C18
Competitor D-C18
Competitor E-C18
As an indicator of column breakdown, retention time of toluene was measured after purging the column with mobile phase. Only the StableBond SB-C18 is unchanged after
three working months of use under these very low pH (0.8) and high temperature (90°C) conditions. ZORBAX Rx-C18 also provides a stable matrix, and can be used as an
alternative selectivity to StableBond SB-C18.
LC and LC/MS
605