TABLE OF CONTENTS - BGB Analytik AG

HPLC Column Protection
Column Protection
Guard columns and in-line filters are inexpensive and easy-to-use tools for column protection.
They can improve the accuracy of the results and improve analytical column lifetime while
enhancing reliability. Column protection is available for all sizes of columns with any particle
size packed into the column.
Guard Columns
Guard columns provide protection against contamination with minimal impact on column
efficiency. Prepacked ZORBAX cartridge columns are available for most types of ZORBAX
material. Guard cartridges are available in different internal diameters to provide high
efficiency protection to all types of columns. Guard columns are also available for many of the
non-Zorbax columns. See the respective column listings for the available guard columns.
Low Volume In-line Filters
Low volume in-line filters are recommended for every column and provide column protection
from particulate materials. An in-line filter will increase analytical column lifetime by
preventing particulates (from unfiltered samples and/or eluents) from plugging the analytical
column frit. Using guard columns can compromise the efficiency of very low volume columns
and/or columns with very small particle sizes. For these columns, low volume in-line filters are
strongly encouraged. A small, 0.5 µm frit should be used to maximize column efficiency.
Replacement Column Inlet Frits
If HPLC columns are used without a guard column on in-line precolumns filter; the analytical
column may become plugged. Due to the high efficiency packing processes used today
replacing the column inlet frit is discouraged. Column efficiency may be compromised if the frit
is replaced. PEEK-encapsulated replacement frits are available for ZORBAX columns packing
in 2.1 mm, 3.0 mm, 4.6 mm, and 9.4 mm standard column hardware.
Replacement Inlet Frits (PEEK Encapsulated) for Standard Hardware
Columns
Description Diameter (mm) Unit Part No.
Narrow Bore 2.1 10/pk 280959-904
Solvent Saver 3.0 1/ea 280959-006
Analytical 4.6 10/pk 280959-905
Semi-Preparative 9.4 1/ea 280959-001
www.agilent.com/chem/supplies
HPLC Column Protection
LC and LC/MS
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