TABLE OF CONTENTS - BGB Analytik AG

Herbicides: Use of Short, Small Particle Columns
for Fast and Efficient Separation
Column A: ZORBAX Rx/SB-C8
866953-906
4.6 x 75 mm, 3.5 µm
Column B: ZORBAX SB-C8
883975-906
4.6 x 150 mm, 5 µm
Mobile Phase: A = Water to pH 2.0 with TFA; B = ACN
Flow Rate: 2.0 mL/min, 1.0 mL/min
Temperature: 35°C
Detector: UV 254 nm
Sample: 0.5 µg each
Separation of EPA 610 PAH Mix
on 3.0 x 250 mm, 5 µm Eclipse PAH Column
Column: Eclipse PAH
959990-318
3.0 x 250 mm, 5 µm
Mobile Phase: A = Water
B = Acetonitrile
Initial %B = 40
Flow Rate: 0.85 mL/min
Gradient: Time (Min)
0.00
17.5
24.0
25.5
27.5
Stop Time = 25.0
Temperature: 25° C
%B
45
100
100
40
40
Detector: 220,4 nm No Ref.; Stop time = 26.0 min
mAU
2000
1500
1000
500
0
A
Relative Absorbance
B
Relative Absorbance
0.3
0.2
0.1
0.0
0.4
0.3
0.2
0.1
0.0
5
0 5 10
Time (min)
0 5
1
2
2
3
1
Rs = 2.2
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3
4
1
5
2
10
4
6 7
5
3
6
8
45
7
Environmental Applications
6
8
7
9
15
Time (min)
15
10 15 20 25 30
Time (min)
8
LCEN013
10 11
1. Tebuthiuron
2. Prometon
3. Prometryne
4. Atrazine
5. Bentazon
6. Propanil
7. Propazine
8. Metolachlor
1. Toluene
2. Naphthalene
3. Acenaphthylene
4. Acenaphthene
5. Fluorene
6. Phenanthrene
7. Anthracene
8. Fluoranthene
9. Pyrene
10. Benzo(a)anthracene
11. Chrysene
12. Benzo(b)fluoranthene
13. Benzo(k)fluoeanthene
14. Benzo(a)pyrene
15. Dibenzo(a,h)anthracene
16. Benzo(g,h,i)perylene
17. Indeno(1,2,3-c,d)pyrene
12
20
13 14
15
16
17
25
LCEPlus
LC and LC/MS
753