TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Food and Consumer Product Applications
Food and Consumer Product Applications
Separation of Azo Dyes on Eclipse Plus Phenyl-Hexyl
Column: Eclipse Plus Phenyl Hexyl
959996-912
4.6 x 100 mm, 5 µm
Mobile Phase: A: 10 mM Ammonium Acetate, pH 4,7
B: MeOH
Flow Rate: 1.5 mL/min
Gradient: Time (Min):
0
5
Detector: UV 254 nm
Anthocyanins from Blueberries:
High-Efficiency High-Speed Separation
Column A: ZORBAX SB-C18
880975-902
4.6 x 250 mm, 5 µm
Column B: ZORBAX SB-C18
863953-902
4.6 x 150 mm, 3.5 µm
Column C: ZORBAX SB-C18
866953-902
4.6 x 75 mm, 3.5 µm
4.6 x 250 mm, 5 µm
4.6 x 150 mm, 3.5 µm
4.6 x 75 mm, 3.5 µm
Mobile Phase: A = 3% Phosphoric acid, B = 100% MeOH
20 30 40 50 60 70
Time (min)
mAU
100
50
1
2
3 4
0
2 3 4 5 6
Time (min)
7 8 9
35 min 26% B
21 min 26% B
A B C
0
10
%B:
25
50
Time Percent B Time Percent B Time Percent B
0 min 23% B
0 min 23% B
0 min 23% B
10.5 min 26% B
97 min 60% B
58.2 min 60% B
29.1 min 60% B
0 5
1. Aniline
2. o-Toluidine
3. Anisidine
4. Benzidine
5. Chloroaniline
6. o-Tolidine
7. Dimethoxybenzidine
8. Npahthylamine
9. Dichlorobenzidine
Flow Rate: 1.0 mL/min
Gradient: As shown
Temperature: 30°C
Detector: UV 525 nm
Sample: Natural Anthocyanins
10 15 20 25 30 35 40
Time (min)
758 www.agilent.com/chem/supplies
5
0 5
6
7
8
10 15
Time (min)
9
20
LCAZO
LCFC001