TABLE OF CONTENTS - BGB Analytik AG

BSA Tryptic Digest on RRHT
Column: ZORBAX SB-C18
820700-902
2.1 x 150 mm, 1.8 µm
Mobile Phase: A: 0.1% TFA, 5% ACN
B: 0.08% TFA, 95% ACN
Flow Rate: 0.5 mL/min
Gradient: A: Time 0 %B 5, Time 30 %B 60
B: Time 0 %B 5, Time 45 %B 60
C: Time 0 %B 5, Time 67.5 %B 60
Temperature: 80°C
Detector: UV 214 nm
Sample: BSA Tryptic Digest
Dextrans Characterization using GPC
A
mAU
40
30
20
10
0
-10
B
mAU
40
30
20
10
0
-10
mAU
40
30
20
10
0
-10
Column: ZORBAX SIL PSM 60
880957-801
6.2 x 250 mm, 5 µm
Column: ZORBAX SIL PSM 300
880957-805
6.2 x 250 mm, 5 µm
Mobile Phase: 100 mM Sodium Acetate (pH 6.0-6.5) with H3PO4
Flow Rate: 0.5 mL/min
Temperature: 30°C
Detector: RI
Sample: Dextrans
C
5
5
5
10 15
10 15
10 15
5
www.agilent.com/chem/supplies
BioPharmaceutical Applications
20
Time (min)
25 30 35 40
20
Time (min)
25 30 35 40
20
Time (min)
25 30 35 40
Dextran 50
43.5k
Dextran 25
21.4k
Dextran 12
9.89k
10 15
Time (min)
Dextran 5
4.44k
Dextran 1
1.08k
20 25
LCCI003
LCBP049
LC and LC/MS
741