TABLE OF CONTENTS - BGB Analytik AG

GC and GC/MS
Life Science Applications
Fentanyls
Column: DB-1701
125-0732
30 m x 0.53 mm, 1.00 µm
Carrier: Hydrogen at 15 mL/min
Oven: 270°C isothermal
Injection: Split, 250°C
Split ratio 1:5
Detector: FID, 300°C
Nitrogen makeup gas at 30 mL/min
Sample: 0.8 µL
Suggested Supplies
Septum: 11 mm Advanced Green septa, 5183-4759
Liner: Split, single taper, low pressure drop, glass wool, 5183-4647
Seal: Gold plated seal, 18740-20885
Syringe: 5 µl tapered, FN 23-26s/42/HP, 5181-1273
Tocopherols
Column: DB-17ms
122-4732
30 m x 0.25 mm, 0.25 µm
Carrier: Helium at 40 cm/sec,
measured at 150°C
Oven: 300°C for 1 min
300-320°C at 25°/min
320°C for 4 min
Injection: Split, 310°C
Split ratio 1:25
Detector: MSD, 310°C transfer line
full scan at m/z 45-550
Sample: 1 µL of 1-10 ng/µL in isooctane
Suggested Supplies
Septum: 11 mm Advanced Green septa, 5183-4759
Liner: Split, single taper, low pressure drop, glass wool, 5183-4647
Seal: Gold plated seal, 18740-20885
Syringe: 5 µl tapered, FN 23-26s/42/HP, 5181-1273
454 www.agilent.com/chem/supplies
1
1
2
10 min
3
4
1. δ-Tocopherol
2. b-Tocopherol
3. γ-Tocopherol
4. α-Tocopherol
4 5
Time (min)
2
3
5
1. Fentanyl
2. Sufentanyl
3. Carfentanyl
4. Lofentanyl
5. Alfentanyl
GCLS012
4
6
GCLS013