TABLE OF CONTENTS - BGB Analytik AG

High Resolution of 24 Amino Acids
Using ZORBAX Eclipse-AAA Protocol
Column: ZORBAX Eclipse AAA
963400-902
4.6 x 150 mm, 3.5 µm
Mobile Phase: A: 40 mM Na2HPO4, pH 7.8
B: ACN : MeOH : Water,
45:45:10 v/v
Flow Rate: 2 mL/min
Temperature: 40°C
Detector: Fluorescence
Sample: 24 Amino Acids
This high resolution separation of 24 amino acids
is done in 18 minutes. If the Rapid Resolution 4.6
x 75 mm Eclipse AAA column is selected, these
amino acids are resolved in 9 minutes.
Amino Acid Analysis (AAA) Columns and Supplies
Amino Acid Standards, 10 x 1 mL ampoules*
Description Part No.
1 nmol/µL 5061-3330
250 pmol/µL 5061-3331
100 pmol/µL 5061-3332
25 pmol/µL 5061-3333
10 pmol/µL 5061-3334
Amino acids supplement kit
Includes 1 g each of norvaline, sarcosine, asparagine, glutamine, tryptophan,
and 4-hydroxyproline
*Consider shelf-life and buy limited quantities, 5062-2478 ships as 1 g vials
5062-2478
Amino Acid Separations Reagents
Description Part No.
OPA reagent, 10 mg/mL each in 0.4 M borate buffer o-phthalaldehyde (OPA)
and 3-mercaptopropionic acid, 6 x 1 mL ampoules
5061-3335
FMOC reagent, 2.5 mg/mL in acetonitrile, 9-fluorenylmethylchloroformate, 1 mL,
10 ampoules
5061-3337
Borate buffer, 100 mL 5061-3339
DTDPA (Dithiodiproprionic) reagent, for analysis of cysteine, 5 g 5062-2479
1
0 5
2
3
4
1. Asp
2. Glu
3. Asn
4. Ser
5. Gln
6. His
www.agilent.com/chem/supplies
5
6
7
8
9
10
11 12
14
7. Gly
8. Thr
9. Cit
10. Arg
11. Ala
12. Tyr
16 17
18
15
19
20
21
10 15
Time (min)
22 23
13. Cys
14. Val
15. Met
16. Nva
17. Trp
18. Phe
24
19. Ile
20. Leu
21. Lys
22. Hyp
23. Sar
24. Pro
20 25
LCPAH01
LC and LC/MS
679