TABLE OF CONTENTS - BGB Analytik AG

Spearmint Oil
Carrier: A: Helium 25 cm/sec measured at 40°C
B: Hydrogen 47 cm/sec measured at 40°C
Oven: A: 40°C hold 1 min, 5°C/min to 290°C
B: 40°C hold 0.38 min, 13°C/min to 290°C hold 13.09 min
Injection: 250°C, Split 40:1, 1-µL injection
Column A: DB-1
122-1032
30 m x 0.25 mm,
0.25 µm
Column B: DB-1
121-1022
20 m x 0.18 mm,
0.18 µm
Original Method with a DB-1, 30 m x 0.25 mm x 0.25 µm
column and Helium carrier
Faster Method with a high efficiency DB-1,
20 m x 0.1 8mm x 0.18 µm column and Hydrogen carrier
www.agilent.com/chem/supplies
High Efficiency Capillary GC Columns
Using hydrogen as a carrier gas in conjunction with the high efficiency column resulted in an overall speed gain of 61% compared to the the original method.
In addition, the resolution was well maintained throughout the method translation process.
A
B
1
1
2 3
4
5
5
3 4
2
8
67
8
9
6 7
9
13
12
10
11
10
11
16
14 15 17
13
12
16
14 15 17
18
19
19
18
20
20
21
21
23
22
22 23
3 5 7
Time (min)
9 11
24
25
25
26
27
24 26
28
27
29 30
8 10 12 14 16 18 20 22 24 26 28
Time (min)
28 29
30
31
SPEARMINTa
31
SPEARMINTb
1.
2. Sabinene
3.
4. 3-Octanol
5. Myrcene
6.
7. ρ-Cymene
8. 1,8-Cineol
9. Limonene
10. cis-Ocimene
11. trans-Ocimene
12.
13. trans-Sabinene hydrate
14. Terpinolene
15. Linalool
16. 3-Octyl acetate
17. Isomenthone
18. Terpinen-4-ol
19. Dihydrocarvone
20. trans-Carveol
21. l-Carvone
22. trans-Dihydrocarveol acetate
23. cis-Carvyl acetate
24. cis-Jasmone
25.
26.
27.
28.
29. trans-b-Farnesene
30. Germacrene-d
31. Viridiflorol
GC and GC/MS
227