TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Poroshell 300
Poroshell 300 Columns Can Separate Proteins
and Peptides in Seconds
Column: Poroshell 300SB-C18
660750-902
2.1 x 75 mm, 5 µm
Mobile Phase: A: 0.1% TFA in H20
B: 0.07% TFA in ACN
Flow Rate: 3.0 mL/min
Gradient: 5-100% B in 1.0 min
Temperature: 70°C, 260 bar pressure
Detector: 215 nm
Sample: Proteins and Peptides
0 0.5
Time (min)
1.0
This separation of eight polypeptides and proteins is completed in less than 60 seconds. Each peak is sharp and efficient.
Reduce Peptide Map Analysis Time by 90%
with Poroshell 300SB
Column A: Poroshell 300SB-C18
660750-902
2.1 x 75 mm, 5 µm
Column B: ZORBAX 300SB-C18
883750-902
2.1 x 150 mm, 5 µm
Mobile Phase: A: 95% H2O, 5% ACN, 0.1% TFA
B: 5% H2O, 95% ACN, 0.07% TFA
Flow Rate: 1 mL/min
0.208 mL/min
Gradient: 0-100%B = 12 min
0-100%B = 120 min
Temperature: 70°C
Sample: 20 µL (0.22 µg/1 µL)
BSA Tryptic Digest
(15 hours, 70 pmol)
686 www.agilent.com/chem/supplies
A
B
1
2
3
4
5
6
7
8
LCPO001
1 2 3 4
Time (min)
5 6 7 8
10
1. Angiotensin II
2. Neurotensin
3. RNase
4. Insulin
5. Lysozyme
6. Myoglobin
7. Carbonic Anhydrase
8. Ovabumin
20 30 40
Time (min)
50 60 70 80
A single chromatographic run of a protein tryptic digest can require an hour or more to complete. With ZORBAX Poroshell columns, the same complex separation can be
completed in 1/10th the time.
LCPO002