TABLE OF CONTENTS - BGB Analytik AG

Blood Alcohols I (Static Headspace/Split)
Column: DB-ALC1
125-9134
30 m x 0.53 mm, 3.00 µm
Carrier: Helium at 80 cm/sec,
measured at 40°C
Oven: 40°C Isothermal
Sampler: Headspace
Injection: Split, 250°C
Split ratio 1:10
Detector: FID, 300°C
Nitrogen makeup gas
at 23 mL/min
Sample:
Suggested Supplies
Septum: 11 mm Advanced Green septa, 5183-4759
Liner: Direct, 1.5 mm ID, 18740-80200
Seal: Gold plated seal, 18740-20885
Blood Alcohols II (Static Headspace/Split)
Column: DB-ALC1
125-9234
30 m x 0.53 mm, 2.00 µm
Carrier: Helium at 80 cm/sec,
measured at 40°C
Oven: 40°C Isothermal
Sampler: Headspace
Oven: 70°C
Loop: 80°C
Transfer Line: 90°C
Vial Equil. Time: 10 min
Pressurization Time: 0.20 min
Loop Fill Time: 0.20 min
Loop Equil. Time: 0.05 min
Inject Time: 0.1 - 0.2 min
Sample Loop Size: 1.0 mL
Injection: Split, 250°C
Split ratio 1:10
Detector: FID, 300°C
Nitrogen makeup gas
at 23 mL/min
Sample: 0.1% Ethanol,
0.001% Others
Suggested Supplies
Septum: 11 mm Advanced Green septa, 5183-4759
Liner: Direct, 1.5 mm ID, 18740-80200
Seal: Gold plated seal, 18740-20885
www.agilent.com/chem/supplies
1
2
3
Life Science Applications
4
0 0.5 1.0 1.5
Time (min)
2.0 2.5 3.0
2
1
3
5
4
5
6
1. Methanol
6
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. 1-Propanol
6. 1-Propanol
0.5 1.0 1.5
Time (min)
2.0 2.5 3.0
GCLS024
GCLS023
GC and GC/MS
459