TABLE OF CONTENTS - BGB Analytik AG

Dexamethasone, USP Method: Rapid Analysis
Column A: ZORBAX SB-C8
880975-906
4.6 x 250 mm, 5 µm
Column B: ZORBAX Rx/SB-C8
866953-906
4.6 x 75 mm, 3.5 µm
Mobile Phase: A = Water, B = ACN; Isocratic 30% B
Flow Rate: 2.0 mL/min
Temperature: Ambient
Detector: UV 254 nm
Sample: Dexamethasone
10 µ L and 5 µ L, 10 ug/mL
USP Method: Triamcinolone
and Internal Standard, Hydrocortisone
Column: ZORBAX Rx-C18
880967-902
4.6 x 250 mm, 5 µm
Column: ZORBAX SB-C18
880975-902
4.6 x 250 mm, 5 µm
Mobile Phase: Water: MeOH, 40/60
Flow Rate: 2.0 mL/min
Temperature: Ambient
Detector: UV 254 nm
Sample: 10 µ L, 10 ug/mL
1
0 5
Time (min)
2
N = 5477
Rs = 5.06
10
1
0 5
Time (min)
2
A B
0 5 10
Time (min)
N = 8297
Rs = 8.37
1. Triamcinolone
2. Hydrocortisone
www.agilent.com/chem/supplies
10
Pharmaceutical Applications
15
0 5
Time (min)
1
0 5 10
Time (min)
2
N = 4078
Rs = 6.16
10
LCPC046
15
LCPC045
LC and LC/MS
789