TABLE OF CONTENTS - BGB Analytik AG

Rapid Resolution HT Provides Double the Efficiency of Rapid
Resolution Columns
Column A: ZORBAX SB-C18
835975-902
4.6 x 50 mm, 3.5 µm
Column B: ZORBAX SB-C18
827975-902
4.6 x 50 mm, 1.8 µm
Mobile Phase: 25% Water, 75% MeOH
Flow Rate: 1.5 mL/min
Temperature: Ambient
Detector: 254 nm
Increase Peak Capacity with RRHT Columns
Column A: Eclipse XDB-C8
928700-906
2.1 x 100 mm, 1.8 µm
Column B: Eclipse XDB-C18
961753-902
2.1 x 100 mm, 3.5 µm
Mobile Phase: A: H2O
B: ACN
Peak capacity: A: 461
B: 343
Flow Rate: 0.5 mL/min
Gradient: 0.0 min 50% B
20.0 min 100% B
Temperature: 40°C
Detector: UV 254 nm
Sample: Alkylphenones
A 2
B
0
ZORBAX Rapid Resolution High Throughput 1.8 µm
1
100
80
60
40
20
0
A
mAU
120
100
80
60
40
20
0
3
Time (min)
1
1
Plates (N)
1. 3476
2. 4585
3. 5673
4. 6180
www.agilent.com/chem/supplies
4
1
1. Uracil
2. Phenol
3. 4-CI-Nitrobenzene
4. Toluene
0.0
1
2
Plates (N)
1. 6560
2. 8958
3. 11508
4. 12266
3
0.5
Time (min)
This figure shows that Rapid Resolution HT columns can provide double the efficiency of a 3.5 µm column in the same column length. This high efficiency can be used for
very high-resolution, high throughput analyses.
0 2 4 6 8 10
Time (min)
12 14 16 18
11
11
4
1.0
LCRR004
LCRR002
0 2 4 6 8 10
Time (min)
12 14 16 18
1. Uracil
5. C6-Alkylphenone 9. C10-Alkylphenone
2. C3-Alkylphenone 6. C7-Alkylphenone 10. C12-Alkylphenone
B
mAU
3.
4.
C4-Alkylphenone
C5-Alkylphenone
7.
8.
C8-Alkylphenone
C9-Alkylphenone
11. C14-Alkylphenone
LC and LC/MS
641