TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
ZORBAX Eclipse XDB
Separation of Cephalosporins on Eclipse XDB-C8
Column: Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
Mobile Phase: 85% 25 mM
Na2HPO4 pH 7: 15% ACN
Flow Rate: 1.0 mL/min
Temperature: 35°C
Sample: Cephalosporins
Selectivity for Urea Pesticides
Column A: Eclipse XDB-C18
993967-902
4.6 x 150 mm, 5 µm
Column B: Eclipse XDB-CN
993967-905
4.6 x 150 mm, 5 µm
Column C: Eclipse XDB-C18
993967-902
4.6 x 150 mm, 5 µm
Mobile Phase: A. 60:40 MeOH:Water
B. 60:40 MeOH:Water
C. 77:23 MeOH:Water
Flow Rate: 1.0 mL/min
Temperature: 25°C
Sample: Urea pesticides
1
2
3
4
0 5 10
Time (min)
15 20
600 www.agilent.com/chem/supplies
A
1
2
3
0 10
B
1
4
2
3 4
5
2
3 4
5
1
5
0 2 4 6 8 10
Time (min)
6
6
5
LCEC009
Cephalosporins are a type of antibiotic and many compounds in this family are well separated on the Eclipse XDB-C8 column.
C
0 2 4 6 8 10
Time (min)
1. Ceftazidine
2. Cefachlor
3. Cefoxatime
4. Cefoxitin
5. Cephalothin
20 30 40 50
Time (min)
1. Fenuron
2. Monuron
3. MonoLinuron
4. Diuron
5. Linuron
6. Pencycuron
The Eclipse XDB-CN column reduces retention time and provides good selectivity for Urea pesticides when compared to an Eclipse XDB-C18 column.
6
LCEC010