TABLE OF CONTENTS - BGB Analytik AG

O
O
Si
Si
Silica Support
C18
C18
Novel Bidentate C18-C18 Bonding for Extend C-18
Bonded Phase
ZORBAX 80Å Extend-C18
www.agilent.com/chem/supplies
ZORBAX 80Å Extend-C18
• High efficiency and long life at high pH – up to pH 11.5
• Unique bidentate bonding and double endcapping provides high pH stability
• More efficiency and better peak shape than polymer-based columns
• Improve retention, resolution and peak shape of basic compounds
• High sensitivity for LC/MS separations of peptides
The Agilent ZORBAX Extend-C18 column uses a novel bidentate C18-C18 bonding technology
to make it possible to develop high-resolution separations at high pH with a silica-based
column. At high pH, non-charged basic compounds will not interact with the underlying silica.
The result is high efficiency separations with superior peak shape and improved resolution.
High pH separations are also the best choice for compounds that are more stable or more
soluble in high pH solutions. Some of the mobile phase buffer options for high pH include
triethylamine, pyrrolidine, glycine, borate and ammonium hydroxide. Ammonium hydroxide at
pH 10.5 is an excellent mobile phase modifier for the LC/MS of peptides and small molecules
with improved sensitivity compared with TFA containing mobile phase at low pH. The Extend-
C18 column is stable from pH 2-11.5 with good peak shape for all types of compounds.
Extend-C18 columns also provide an additional selectivity choice at low pH.
Column Specifications
Pore Surface Temp. pH
Carbon
Bonded Phase Size Area Limits* Range** Endcapped Load
ZORBAX Extend-C18 80Å 180 m2/g 60°C 2.0-11.5 Double 12.5%
Specifications represent typical values only.
*Temperature limits are 60°C up to pH 8, 40°C from pH 8-11.5.
**Above pH 6 highest column stability for all silica based column is obtained by reducing the operating temperature to 40°C
or below and using lower buffer concentrations (0.01 - 0.02M) or organic buffers.
LC and LC/MS
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