TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Pharmaceutical Applications
Hydrophilic Purine/Pyrimidine Separation
Column: ZORBAX SB-Aq
883975-914
4.6 x 150 mm, 5 µm
Mobile Phase: 50 mM NaOAc, pH 4.6
Flow Rate: 2.0 mL/min
Temperature: 35°C
Detector: UV 254 nm
mAU
Warfarin: USP Chromatographic Purity Method Using Eclipse XDB-CN
Column: Eclipse XDB-CN
993967-905
mAU
4.6 x 150 mm, 5 µm
8
1. Warfarin (0.128 mg/ml)
Mobile Phase: 32:68:1
Acetonitrile:Water:Glacial
Acetic Acid
Flow Rate: 1.5 mL/min
Temperature: 25ºC
Detector: UV 260 nm
Sample: Warfarin, 2 µL
Ten Cardiac Drugs on Rapid Resolution HT SB-C18
Column: SB-C18
829975-902
4.6 x 150 mm, 1.8 µm
Mobile Phase: A: 0.1% TFA, 5% ACN
B: 0.08% TFA, 95% ACN
Flow Rate: 2 mL/min
Gradient: 0.0 min 12.5% B
10.5 min 60% B
12.0 min 60% B
Temperature: 70°C
Detector: UV 230 nm
Sample: Cardiac Drugs
25
20
15
10
5
0
1
3
2
4
5
0 5
790 www.agilent.com/chem/supplies
6
4
2
0
2. Warfarin Related Compound A (0.118 mg/ml)
10
Time (min)
15 20
0 1 2 3 4 5 6 7 8 9
Time (min)
1
2
3
4
5
6
7
1
1. Procainamide
2. Procaine
3. Nadolol
4. Pindolol
5. Lidocaine
6. Disopyramide
7. Propranolol
8. Nifedipine
9. Nimodipine
10. Nisoldipine
1. Cytosine
2. Fluorocytosine
3. Uracil
4. Guanine
5. Thymine
6. Adenine
0.5 1.0 1.5 2.0
Time (min)
2.5 3.0 3.5 4.0
8
2
9
10
LCPC048
LCPC047
LCPC049