TABLE OF CONTENTS - BGB Analytik AG

GC and GC/MS
Life Science Applications
Free Steroids
Column: DB-17
122-1731
30 m x 0.25 mm, 0.15 µm
Carrier: Hydrogen at 44 cm/sec
Oven: 260°C isothermal
Injection: Split, 250°C
Split ratio 1:100
Detector: FID, 300°C
Nitrogen makeup gas at
30 mL/min
Sample: 1 µL
Anabolic Steroids
Column: DB-1
122-1031
30 m x 0.25 mm, 0.10 µm
Carrier: Helium at 40 cm/sec, measured at 180°C
Oven: 180-320°C at 10°/min
320°C for 4 min
Injection: Split ratio 1:40
Detector: FID, Nitrogen makeup gas at 30 mL/min
Sample: 2 µL of 0.125 µg/µL each in methanol
458 www.agilent.com/chem/supplies
1 3 5
4
2
6, 7
18 min
10, 11
12
8 9
13
14
15 16
1
2
3
4
5
17
GCLS021
6
7
8
18 min
9
10
1. Coprostane (5-ß-cholestane)
2. 5-b-Androsterone
3. 5-α-Cholestane
4. Androsterone
5. Epiandrosterone (trans-androsterone)
6. 17-α-Estradiol
7. b-Estradiol
8. Estrone
9. Progesterone
10. Cholesterol
11. Estriol
12. Stigmasterol
11
12
GCLS020
1. Dehydroisoandrosterone (Prasterone)
2. 5α-Androstan-17α-ol-3-one (Stanolone)
3. 19-Nortestosterone (Nandrolone)
4. Mesterolone
5. Testosterone
6. 1-Dehydrotestosterone (Boldenone)
7. 17α-Methyltestosterone
8. 1-Dehydro-17-α-methyltestosterone (Methandrostenolone)
9. Norethandrolone
10. 1-Dehydrotestosterone acetate
11. Oxymetholone
12. 19-Nortestosterone-17-propionate
13. 4-Chlortestosterone-17-acetate (Clostebal)
14. Stanozolol
15. 1-Dehydrotestosterone benzoate
16. 19-Nortestosterone-17-decanoate
17. 1-Dehydrotestosterone undecylenate