TABLE OF CONTENTS - BGB Analytik AG

Triton X-114: Decreasing Run-time by Changing Bonded Phase
Column A: ZORBAX SB-C3
883975-909
4.6 x 150 mm, 5 µm
Column B: ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
Mobile Phase: MeOH and H2O (as indicated)
Flow Rate: 1.0 mL/min
Temperature: 50°C
Detector: UV 225 nm
Sample: Triton X-114
PMMA Polymer with MW = 316000
Column: PLgel mixed C
79911GP-MXC
7.5 x 300 mm, 5 µm
Mobile Phase: Tetrahydrofuran (THF)
Flow Rate: 1.0 mL/min
Temperature: 20°C
Detector: RI and DAD
Sample: 26 mg dissolved in
1 mL THF, 10 µL
Calibrant: Polystyrene EasyCal
Vial standard (5064-8281)
Norm
300
250
200
150
100
50
0
A
0 2 4 6 8 10 12 14 16
Time (min)
B
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
Time (min)
DAD signal
15
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Chemical/Industrial Applications
Refractive
index signal
ZORBAX SB-C3
70/30% MEOH/H2O
ZORBAX SB-C18
75/25% MEOH/H2O
LCCI010
20 25 30
Time (min)
LCCI011
LC and LC/MS
745