TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
ZORBAX Rapid Resolution 3.5 µm
Run Method Development Chromatograms
on Rapid Resolution Columns
Column: ZORBAX SB-C18
866953-902
4.6 x 75 mm, 3.5 µm
Mobile Phase: A: 25 mM NaH2PO4, pH 3.0
B: MeOH
A: 20:80
B: 30:70
C: 40:60
Flow Rate: 2.0 mL/min
Temperature: 35°C
Detector: 254 nm
A B C
1
1
2 3
0.0 0.5 1.0 1.5 2.0 2.5 3.0 0.0
Time (min)
648 www.agilent.com/chem/supplies
5
2 3
23 4
4
0.5
1. Diltiazem
2. Dipyridamole
3. Nifedipine
4. Lidoflazine
5. Flunarizine
1.0 1.5 2.0 2.5 3.0
Time (min)
0.0
0.5
1
1.0 1.5 2.0 2.5 3.0
Time (min)
Rapid Resolution 4.6 x 75 mm, 3.5 µm columns are a good choice for initial method development because they allow you to quickly determine if a given column and mobile
phase combination can produce the desired separation.
ZORBAX Rapid Resolution 3.5 µm Eclipse Plus Columns
Hardware Description Size (mm)
*RR: Rapid Resolution 3.5 µm
Particle Size
(µm)
Eclipse
Plus C18
USP L1
Eclipse
Plus C8
USP L7
5
LC3_502
Eclipse Plus
Phenyl Hexyl
USP L11
Rapid Resolution 4.6 x 150 3.5 959963-902 959963-906 959963-912
Rapid Resolution 4.6 x 100 3.5 959961-902 959961-906 959961-912
Rapid Resolution 4.6 x 75 3.5 959933-902 959933-906 959933-912
Rapid Resolution 4.6 x 50 3.5 959943-902 959943-906 959943-912
Rapid Resolution 4.6 x 30 3.5 959936-902 959936-906 959936-912
Solvent Saver Plus 3.0 x 150 3.5 959963-302 959963-306 959963-312
Solvent Saver Plus 3.0 x 100 3.5 959961-302 959961-306 959961-312
Narrow Bore RR* 2.1 x 150 3.5 959763-902 959763-902 959763-912
Narrow Bore RR* 2.1 x 100 3.5 959793-902 959793-906 959793-912
Narrow Bore RR* 2.1 x 50 3.5 959743-902 959743-906 959743-912
Narrow Bore RR* 2.1 x 30 3.5 959733-902 959733-906 959733-912
Guard Cartridges, 4/pk 4.6 x 12.5 5 820950-936 820950-937
Guard Cartridges, 4/pk 2.1 x 12.5 5 821125-936 821125-937
Guard Hardware Kit 820888-901 820888-901