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TABLE OF CONTENTS - BGB Analytik AG

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LC and LC/MS<br />

Cartridge Column Systems<br />

This icon identifies prep preparative cartridge and<br />

guard-columns.<br />

Guard Cartridge, 820444-901<br />

Assembled 420420-901<br />

Preparative External Guard Hardware Kit.<br />

582 www.agilent.com/chem/supplies<br />

ZORBAX PrepHT and Agilent Prep Preparative Cartridge<br />

and Guard-Column Hardware<br />

The ZORBAX PrepHT and Agilent Prep Preparative Cartridge and Guard-Column hardware kits<br />

have been developed to provide a convenient preparative 21.2 mm ID cartridge design. The<br />

21.2 mm ID preparative cartridge columns (actual ID 17 mm to fit into holder) are reusable<br />

and allow rapid change of column lengths from 50-250 mm for optimizing sample loadability.<br />

This easy-to-use cartridge hardware design is used for both ZORBAX PrepHT and Agilent Prep<br />

materials and can be finger-tightened up to 5000 psi (350 bar).<br />

The cartridge hardware can be used stand-alone or with an integral guard column. The<br />

integral guard column holder is a stainless steel body and is used with a Teflon sealing gasket<br />

to assure a tight, leak-free and extremely low-dead-volume seal against the 21.2 mm ID<br />

cartridge body. The external guard system seals fingertight up to 2000 psi (135 bar). The<br />

reusable guard holder is ready to use with standard 1/16 in. LC fittings. Both ZORBAX and<br />

Agilent Prep guard cartridges are available to use with this holder and are selected to match<br />

the preparative column used in the application.<br />

The 21.2 mm ID guard columns can be used with the 30 mm ID Agilent Prep columns. For this<br />

application, select the external preparative guard-column hardware kit.<br />

PrepHT Columns are easy to use<br />

Column Cartridge<br />

Standard Endfitting<br />

Polymeric Seal<br />

Seal Holder and Seal Pusher<br />

Guard Column Endfitting<br />

Guard Column<br />

Polymeric Seals<br />

PrepHT cartridge columns have a unique design that makes them easy to install and seal finger tight up to 5000 psi.<br />

The cartridge design allows for an integral guard column to be used, which prolongs the life of the purification column.<br />

This cartridge configuration is economical to use since the column cartridge and/or the guard cartridges are replaced<br />

independently. The endfittings are used many times.<br />

Hardware<br />

Description Part No.<br />

PrepHT cartridge column hardware<br />

820400-901<br />

Includes cartridge column end fittings (2), polymeric seals (2)<br />

PrepHT guard-column hardware kit<br />

820444-901<br />

Includes guard column end fitting, polymeric seal, and seal insertion tool<br />

(seal holder and seal pusher)<br />

Agilent Prep external guard hardware kit, Includes guard holder, guard column 420420-901<br />

end fitting, polymeric seal (2), seal insertion tool, and connector tubing<br />

Replacement polymeric seals, 2/pk 820385-901

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