TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Pharmaceutical Applications
Aspirin and Cough Remedy on ZORBAX Eclipse XDB-C8
Column: Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
Mobile Phase: (75:25) 25 mM Na2HPO4 (pH 3.0): ACN
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detector: UV 254 nm
Sample: 5 µL, 10 µg/mL
Guaifenesin: USP Analysis of Guaifenesin
Mobile Phase: 40% Methanol:60% Water:1.5% Glacial Acetic Acid
Flow Rate: 1.0 mL/min
Temperature: 25°C
Sample: Guaifenesin
a: 8 µL
b: 2mL
780 www.agilent.com/chem/supplies
LCPC025
1
0 1 2 3 4 5 6 7 8 9 10
Time (min)
Column: Eclipse XDB-C18
990967-902
4.6 x 250 mm, 5 µm
1
2
LCPC024
1. Acetylsalicylic acid
2. Dextromethorphan
0 2
4
6
Time (min)
8 10 12
0
1. Guaifenesin: 0.04 mg/mL
2. Benzoic Acid: 0.10 mg/mL
1 2
Time (min)
3
Column: Eclipse XDB-C18
922975-902
4.6 x 50 mm, 1.8 µm
Minimum Resolution Required = 3.0
Peak TR N Rs
1 6.63 12,737 0
2 11.19 18,552 15.8
Peak TR N Rs
1 1.4 11,421 0
2 2.33 12,909 12.3
2