TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Food and Consumer Product Applications
Patuline in Apple Juice
Column: LiChrospher 100 Diol
79925DI-564
4 x 125 mm, 5 µm
Mobile Phase: Hexane-isopropanol 95:5 as isocratic mixture
Flow Rate: 0.6 mL/min
Temperature: 30°C
Detector: DAD 270/20 nm
Confirmation: spectral information
and retention times
Sample: a: Apple juice
b: Standard
766 www.agilent.com/chem/supplies
mAU
Fat-Soluble Vitamins: Separation of Vitamin D2 from D3
Column A: ZORBAX ODS
884950-543
4.6 x 250 mm, 5 µm
Column B: ZORBAX ODS
883952-702
4.6 x 150 mm, 5 µm
Mobile Phase: 75/25 ACN/MeOH
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detector: UV 325 nm for 4 min, 280 nm after
Sample: 5 µL (10 µg/µL), Fat Soluble Vitamin Standard
Fat-Soluble Vitamins on ZORBAX Eclipse XDB-C8
Column: Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
Mobile Phase: 5/95 Water/MeOH
Flow Rate: 1.0 mL/min
Temperature: a: Ambient
b: 50°C
Detector: UV 280 nm
Sample: Fat Soluble Vitamins
30
20
10
0
HMF
Patuline
Patuline
Apple Juice
Standard
0 2 4 6 8
Time (min)
10 12 14
0
1
2
0 5
3
1 2
1 2
4
5
6
10 15 20 25 30
Time (min)
3
3
4 5
7
4 5
6
Scaled
1
2
7
6
5
4
3
2
1
0
3
5
0 5
6
4
Library match 985
2.5 5.0 7.5 10.0
Time (min)
12.5 15.0 17.5
Ambient 50°C
6
7
7
8
8
985
250
300 350
Wavelength (nm)
7
1. all-Trans retinol
2. all-Trans acetate
3. (+)-d-Tocopherol
4. Vitamin D2
5. Vitamin D3
LCFC023
10 15 20 25 30
Time (min)
LCFC024
LCFC021
6. (+)-a-Tocopherol (Vitamin E)
7. (+)-a-Tocopherol Acetate
8. Vitamin K1
1. Retinol
2. Retinol acetate
3. Vitamin D3
4. γ-Tocopherol
5. α-Tocopherol
6. Tocopherol acetate
7. Retinol palmitate