TABLE OF CONTENTS - BGB Analytik AG

GC and GC/MS
Life Science Applications
Marijuana (D9-THC) and Major Metabolites - TMS Derivatives
Column: DB-5
123-5032
30 m x 0.32 mm, 0.25 µm
Carrier: Helium at 40 cm/sec, measured at 100°C
Oven: 100°C for 1 min
100-175°C at 30°/min
175-295°C at 12°/min
Injection: Splitless, 250°C
30 sec purge activation time
Detector: FID, 300°C
Nitrogen makeup
gas at 30 mL/min
Sample: 1 µL of 0.1 µg/µL each in pyridine
Suggested Supplies
Septum: 11 mm Advanced Green septa, 5183-4759
Liner: Direct connect, single taper, deactivated, 4 mm ID, G1544-80730
Seal: Gold plated seal, 18740-20885
Syringe: 10 µl tapered, FN 23-26s/42/HP, 5181-1267
Blood Pollutants I
Column: DB-ALC1
125-9134
30 m x 0.53 mm, 3.00 µm
Carrier: Helium, 36 cm/sec, measured at 40°C
Oven: 40°C for 5 min
40-210°C at 10°/min
Injection: Split, 250°C
Split ratio 1:10
Detector: FID, 300°C
Suggested Supplies
Septum: 11 mm Advanced Green septa, 5183-4759
Liner: Direct, 1.5 mm ID, 18740-80200
Seal: Gold plated seal, 18740-20885
460 www.agilent.com/chem/supplies
1*
2*
3*, 4
1. Methanol*
2. Acetaldehyde*
3. Ethanol*
4. Diethyl ether
5. Isopropyl alcohol*
6. Methylene Chloride
7. Acetone*
8. Acetonitrile
9. Ethyl formate
10. t-Butyl alcohol*
5*
7*, 8, 9, 10*
6
11*
12
13
14
15
16
17, 18
11. 1-Propanol
12. MTBE
13. Hexane
14. Chloroform
15. sec-Butyl alcohol
16. 2-Chlorobutane
17. MEK (2-Butanone)
18. Ethyl acetate
19. 1,1-Trichloroethane
20. Carbon tetrachloride
21. 1-Chlorobutane
22. Benzene
23. 1-Butanol
24. Heptane
19, 20
21, 22
23
1. Δ9-THC
2. L-11-Hydroxy-Δ9-THC
3. L-9-Carboxy-11-nor-Δ9-THC
24
1 2 3 4 5 6 7 8
Time (min)
9 10 11 12 13 14 15
25
26
13.5 min
25. Ethylene glycol
26. soamyl alcohol
27. Toluene
28. Isopropyl amine (not shown)
29. Ethylbenzene
30. m,p-Xylene
31. o-Xylene
30
32. DMSO
27
28, 29
1 2
31
32
3
GCLS025
GCLS022