TABLE OF CONTENTS - BGB Analytik AG

Water-Soluble Vitamins
Column: ZORBAX SB-C8
883975-906
4.6 x 150 mm, 5 µm
Mobile Phase: A = 50 mM Sodium Phosphate, pH 2.5/MeOH (90/10)
B = 50 mM Sodium Phosphate, pH 2.5/MeOH (10/90)
Flow Rate: 1.0 mL/min
Gradient: 0-70% B in 18 min
Temperature: Ambient
Detector: UV 245 nm
Sample: Water soluble vitamins
Water-Soluble Vitamins:
High Speed Separation using Ion-Pairing
Column: ZORBAX Rx/SB-C8
866953-906
4.6 x 75 mm, 3.5 µm
Mobile Phase: 10 mM Hexane Sulfonate with 0.1%
Phosphoric Acid: MeOH (74:26)
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detector: UV 245 nm
Sample: Water soluble vitamins
Water-Soluble Vitamins using the USP 23 Method
Column: ZORBAX SB-C18
880975-902
4.6 x 250 mm, 5 µm
Mobile Phase: 7.2 mM Hexane Sulfonate/MeOH/Acetic Acid
(73/27/1) (ratio to 101)
Flow Rate: 1.0 mL/min
Temperature: 30° C
Detector: UV 280 nm
Sample: Water soluble vitamins
0
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Food and Consumer Product Applications
0
1
2
3
4
5
2.5 5.0 7.5
Time (min)
10.0 12.5
1
2
3
4
5
6
7
6
8
LCFC026
0 1 2 3
Time (min)
4 5
1
1
E
2
2
1. B1-Thiamine
2. Vitamin C
3. B3-Niacin
4. B6-Pyridoxine
5. Pantothenic acid
6. Folic acid
7. B12-Cyanocobalamin
8. B2-Riboflavin
1. Vitamin C
2. B3-Niacin
3. B6-Pyridoxine
4. Folic acid
5. B2-Riboflavin
6. B1-Thiamine
LCFC025
USP Standard Preparation
2.5 5.0 7.5 10.0
Time (min)
12.5 15.0 17.5
3
Analysis of Vitamin Formulation
3
4
1. B3-Niacin
2. B6-Pyridoxine
3. B2-Riboflavin
4. B1-Thiamine
E Excipent
4
LCFC027
LC and LC/MS
767