TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Food and Consumer Product Applications
Polysaccharide: Size Separation
Column: ZORBAX PSM Bimodal Kit, 2 columns
880949-903
6.2 x 250 mm, 5 µm
Mobile Phase: 8.5 g NaCl per liter water
Flow Rate: 2.0 mL/min
Temperature: 35°C
Detector: UV 205 nm and RI
Sample: 1. MW = 40,000 polysaccharide (Rheomacrodex)
2. Glucose
Tocopherols: Analysis of Margarine
with Fluorescence Detection
Column: LiChrospher 100 Diol
79925DI-564
4 x 125 mm, 5 µm
Mobile Phase: Hexane + 2% Isopropanol
Flow Rate: 1.0 mL/min
Temperature: 25°C
Detector: Fluorescence, Ex = 295, Em = 330
Sample: a: Standard
b: Margarine
Tocopherols by LC/MS with APPI
Column: Eclipse XDB-C18
993967-302
3.0 x 150 mm, 5 µm
Mobile Phase: 97% MeOH: 3% 10mM CH3COONH4
Flow Rate: 0.5ml/min
Temperature: 40°C
MS Conditions: MS: Agilent 1100MSD SL
Ionization: APPI(Positive)
Scan range: m/z 100-500
Vcap: 1500 V
SIM ion: base peak
Drying gas: 7 l/min at 350°C
Nebulizer gas: 60psi
Vaporizer temp: 350°C
Fragmentor: 140 V
EM gain: 4
Sample Volume: 10 µl
CH3
O
764 www.agilent.com/chem/supplies
RI
UV
0 2 4 6
Time (min)
8 10 12
%F
100
90
80
70
60
50
40
30
20
10
1 (11.2%)
400000
300000
200000
100000
0
H 3C
HO
1
CH 3
H 3C
HO
2
CH3 O
2 (77.3%)
1: alpha-tocopherol
3
HO
CH3
O
2: beta-tocopherol
3: gamma-tocopherol
0 2 4 6 8 10 12 14
Time (min)
1
4
3 (9.5%) 4 (1.9%)
1 2 3
Time (min)
4 5 6
1
2
LCFC009
1. alpha Tocopherol
2. beta Tocopherol
3. gamma Tocopherol
4. delta Tocopherol
2
LCFC012
3
LCFC011