TABLE OF CONTENTS - BGB Analytik AG

Improved Peak Shape of Basic Compounds Using Bonus-RP
Column: Alkyl-C8
4.6 x 150 mm, 5 µm
Mobile Phase: 75% 25 mM NH4OAc, pH 5.5
25% ACN
Flow Rate: 1.5 mL/min
Temperature: 40°C
Detector: 254 nm
TF
1. 1.8
2. 1.7
3. 2.1
1
0 1 2 3 4 5 6 7 8 9
Time (min)
Column: ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm
Mobile Phase: 80% 25 mM NH4OAc, pH 5.5
20% ACN
Flow Rate: 1.5 mL/min
Temperature: 40°C
Detector: 254 nm
TF
1. 1.1
2. 1.1
3. 1.1
1
0 1 2 3 4 5 6
Time (min)
2
2
1. Doxylamine
2. Chlorpheniramine
3. Triprolidine
3
3
10
LCBR001
LCBR002
1. Doxylamine
2. Chlorpheniramine
3. Triprolidine
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ZORBAX Bonus-RP
Bonus-RP eliminates peak tailing of these basic compounds in comparison to a typical alkyl C8 bonded phase. In the mid-pH region, residual silanols can interact more
strongly with basic compounds to cause peak tailing. The polar group in the Bonus-RP bonded phase eliminates peak tailing of these basic compounds by reducing
interactions with residual silanols.
LC and LC/MS
613