TABLE OF CONTENTS - BGB Analytik AG

Novel Bidentate C18-C18 Bonding for Extend-C18
Bonded Phase
ZORBAX 300Å Extend-C18
• Rugged, high and low pH separations of polypeptides and peptides from pH 2-11.5
• Different selectivity possible at high and low pH
• High efficiency and good recovery of hydrophobic peptides at high pH
• Ideal for LC/MS with ammonium hydroxide-modified mobile phase
Agilent ZORBAX 300Extend C-18 is a wide-pore HPLC column for high efficiency separations
of peptides from pH 2-11.5. The unique, bidentate bonded phase provides excellent lifetime
and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and
polypeptides can change dramatically as a result of changes in charge on molecules. Excellent
recoveries of hydrophobic polypeptides have been achieved at room temperature and high
pH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a
simple ammonium hydroxide-containing mobile phase.
Column Specifications
Pore Surface Temp.
Carbon
Bonded Phase Size Area Limits* pH Range Endcapped Load
ZORBAX 300Extend-C18 300Å 45 m2/g 60°C 2.0-11.5 Double 4%
Specifications represent typical values only.
*Temperature limits are 60°C up to pH 8, 40°C from pH 8-11.5.
www.agilent.com/chem/supplies
ZORBAX 300Å Extend-C18
LC and LC/MS
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