TABLE OF CONTENTS - BGB Analytik AG

Peptide Map – Electronic Flow
Control Reproducibility of 10
Injections on Capillary LC Column
Column: ZORBAX 300SB-C18
5064-8265
0.3 x 250 mm, 5 µm
Mobile Phase: A - 0.05% TFA in Water
B - 0.045% TFA in Acetonitrile
Flow Rate: 5.5 µL/min.
Gradient: 0.5% B/min.
Temperature: 30°C
Detector: UV 206/10 nm, 450/80 nm
Sample: 1.3 µL (myoglobin tryptic digest,
7.5 pmol/µL)
0.28
mAU
1400
1200
1000
800
600
400
200
Capillary LC/ESI-MS Concentration Effects
on Detection of a Protein Digest
Column A: ZORBAX 300SB-C18
881750-902
2.1 x 250 mm, 5 µm
Column B: ZORBAX 300SB-C18
861630-902
1.0 x 250 mm, 5 µm
Column C: ZORBAX 300SB-C18
5064-8265
0.3 x 250 mm, 5 µm
Mobile Phase: A– 0.1% TFA in water
B– 0.1% TFA in ACN
Flow Rate:
Gradient: 0-60% B/60 min.
Temperature:
Detector:
MS Conditions: TIC 300-3000 m/z
Publication:
Sample: 6.6 pmol (5 µL) of LysC digest of DSPA
0
TIC 300-3000 m/z
0.09
0.10
mAU
200
150
100
0.09
0.11
0.11
www.agilent.com/chem/supplies
50
BioPharmaceutical Applications
40.0 40.5 41.0 41.5 min
0.12
20 30 40 50 60 70
Time (min)
10000
30000
A
B
C
600000
0.11
0.11
0.11
0.12
20 30 40
20 30 40
20 30
Time (min)
40
RSD RT
0.13
LCBP028
LCBP029
LC and LC/MS
733