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TABLE OF CONTENTS - BGB Analytik AG

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Good Peak Shape Over a Wide pH Range<br />

with ZORBAX Eclipse XDB<br />

Column: Eclipse XDB-C8<br />

993967-906<br />

4.6 x 150 mm, 5 µm<br />

Mobile Phase: A:<br />

pH 3.0 75% 25 mM<br />

phosphate buffer<br />

pH 3.0: 25% ACN<br />

B:<br />

pH 7.0 90% 20 mM<br />

phosphate pH 7.0<br />

10% ACN<br />

Flow Rate: 1.5 mL/min<br />

Temperature: 40°C<br />

Sample: A:<br />

1. Maleate<br />

2. Doxylamine<br />

3. Chlorpheniramine<br />

4. Triprolidine<br />

5. Diphenhydramine<br />

B:<br />

1. Procainamide<br />

2. N-acetylprocainamide<br />

3. N-propionylprocainamide<br />

ZORBAX Eclipse XDB columns provide good peak shape over a<br />

wide pH range and are an excellent choice for method<br />

development from pH 2-9.<br />

A B<br />

2<br />

1<br />

3<br />

www.agilent.com/chem/supplies<br />

4<br />

0 2.5 5.0<br />

Time (min)<br />

7.5<br />

5<br />

ZORBAX Eclipse XDB<br />

1<br />

2<br />

0 2.5 5.0<br />

Time (min)<br />

7.5<br />

3<br />

LCEC004<br />

LC and LC/MS<br />

597

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