TABLE OF CONTENTS - BGB Analytik AG

Column Specifications
Multiple Affinity Removal System – Human 7 LC Columns
Description Serum Capacity Per Injection Part No.
Hu-7, 4.6 x 50 mm 30 - 35 µL 5188-6409
Hu-7, 4.6 x 100 mm 60 - 70 µL 5188-6410
Hu-7, 10 x 100 mm 250 - 300 µL 5188-6411
Load capacities based on pooled normal human plasma standard.
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Multiple Affinity Removal LC Columns
Multiple Affinity Removal System –
Human 7 LC Columns
The Multiple Affinity Removal System - Human 7 LC Column removes seven interfering highabundant
proteins from human serum, plasma and other biological fluids, which improves
LC/MS and electrophoretic analysis.
• Eliminates 98% or greater of each of the 7 targeted high-abundant human proteins
• Sample loss minimized with rapid, single injection protocols and optimized buffers
• Two buffer system allows re-use of the columns for over 200 injections
• Compatible with most FPLC or HPLC pumps or instruments, including
Agilent 1100/1200 Series
Dimensions 4.6 x 50 mm (0.83 mL bed volume)
4.6 x 100 mm (1.66 mL bed volume)
10 x 100 mm (7.8 mL bed volume)
Custom sizes available
Body material PEEK (polyetheretherketone)
End fitting material PEEK with 2 µm frits
Injections/Column > 200
Total serum/plasma processed per lifetime 4.6 x 50 mm: 7,000 µL
4.6 x 100 mm: 14,000 µL
10 x 100 mm: 60,000 µL
Maximum pressure 120 bar
Operating temperature 18 - 25°C
Packing material Affinity resin
Immobilized ligands Affinity ligands to human (albumin, IgG, IgA,
transferrin, haptoglobin, antitrypsin, and
fibrinogen)
Flow rate range 0.25 - 3.0 mL/min
Shipping solution Buffer A with 0.02% sodium azide
Shipping temperature 2 - 8°C
Storage temperature 2 - 8°C
LC and LC/MS
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