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LC and LC/MS ZORBAX Ion Exchange Columns – SAX and SCX ZORBAX Ion Exchange Columns – SAX and SCX • ZORBAX SAX and 300SCX columns are based on rugged ZORBAX silica • Stable from pH 2-7 • Provide high efficiency, rapid separations • Compatible with organic mobile phase modifiers Agilent ZORBAX Strong Ion Exchange columns are available as both Strong Anion Exchange (SAX) and Strong Cation Exchange (300SCX) columns. Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency. Column Specifications Bonded Max Phase Pore Size Surface Area pH Range Functionality Pressure ZORBAX SAX 70Å 300 m2/g 2.0-7.0 Quaternary amine 350 bar ZORBAX 300SCX 300Å 50 m2/g 2.0-7.0 Sulfonic acid 350 bar Specifications represent typical values only. 634 www.agilent.com/chem/supplies ZORBAX SAX packing has a permanently bonded quaternary amine. A trifunctional organosilane reagent is used in producing this packing to maximize its stability with aqueous mobile phases. This column is ideal for separation of water-soluble compounds such as aromatic and aliphatic carboxylic acids and sulfonic acids. ZORBAX SCX packing has 300Å pore size silica particles chemically bonded to an aromatic sulfonic acid group. This column is used for separations of basic, water-soluble compounds and bio-molecules.
Cough-Cold Remedies - ZORBAX 300SCX Column: ZORBAX 300ÅSCX 880952-704 4.6 x mm, 5 µm Mobile Phase: 100 mM NaH2PO4 (pH 6.5) Flow Rate: 3 mL/min Temperature: 20° C Detector: 210 nm Sample: Cold remedies ZORBAX Ion Exchange Columns – SAX and SCX 1 2 3 4 0 2 Time (min) 4 LCIE001 ZORBAX Ion Exchange Columns – SAX and SCX Description Size (mm) www.agilent.com/chem/supplies 5 1. Pyrilamine 2. Theophylline 3. Glyceryl Guaicolate 4. Caffeine 5. Phenylephrine Particle Size (µm) SAX 300SCX Semi-preparative 9.4 x 250 5 880952-203 880952-204 Analytical 4.6 x 250 5 880952-703 880952-704 Analytical 4.6 x 150 5 883952-703 883952-704 Analytical 4.6 x 50 5 846952-704 Solvent Saver 3.0 x 50 5 860700-304 Narrow Bore 2.1 x 150 5 883700-704 Narrow Bore 2.1 x 50 5 860700-704 Capillary 0.3 x 35 3.5 Capillary 0.8 x 50 3.5 Guard Cartridge, 4/pk 4.6 x 12.5 6 820950-903 820950-904 Guard Hardware Kit 820888-901 820888-901 LC and LC/MS 635
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TABLE OF CONTENTS Featured Products
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7683B Automatic Liquid Sampler Deli
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Number of pesticide hits Number of
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Agilent 1200 Series LC Systems and
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1200 Series Purification Systems De
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Agilent 6300 Series Ion Trap LC/MS
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Make the Change to ICP-MS with Agil
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BioCel Automation Systems Through i
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Stratagene Real-time PCR Solutions
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MS Certified Liners www.agilent.com
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Focus on what you do best, and leav
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Agilent Offices Worldwide Australia
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Brazil Hexis Científica Jundiaí,
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Japan Yokogawa & Co., Ltd. 7-336, K
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Thailand Worldco Co. Ltd 63/3 Arun-
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General Chromatography Supplies Con
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Agilent Vial Identification Chart a
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Screw top vials with fixed inserts
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Preassembled vial pack Pre-Assemble
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Magnetic crimp caps for CTC, 5188-5
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Snap caps with septa Snap Top Caps
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Polypropylene Crimp/Snap Top Vials
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Micro-V Vials • Wide opening, 1.5
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8-425 screw top vials 8-425 Screw T
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4 mL screw top vials 4 mL Screw Top
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Certified headspace crimp top vials
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General Purpose Sample and Storage
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Manual crimper and decapper Micro v
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Syringes With a broad selection of
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Straight Needle, 23 and 26s Gauge A
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10 µl fixed needle 25-500 µL fixe
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On-column syringe Push pull Push bu
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CTC Analytics PAL Autosampler Suppl
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Fixed needle, G6500-80109 CombiPAL
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X-type fixed needle, G4200-80118 Re
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Sample tray, G6500-80101 Well plate
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Carrier Gas Purification Gas supply
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Gas Purifier Selection Guide In Lin
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a b (Figures a and b. The “plus
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Triple filter cartridge, 5182-9705
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Gas Traps www.agilent.com/chem/supp
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Molecular Sieve 5A and Indicating D
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Moisture S-trap Big moisture trap M
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Big oxygen trap Big hydrocarbon tra
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Capillary Grade Hydrocarbon Traps (
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Hydrocarbon Moisture trap Hydrocarb
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Split vent trap and cartridges, RDT
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ADM 1000 ADM 2000 ADM 1000 www.agil
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Low Gas Alarm System, 2.0 and 2.5 i
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www.agilent.com/chem/supplies Regul
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Fittings kit, 5180-4161 GC Installa
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Brass adapter, 0100-0420 Stainless
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Reducing union, 0100-0241 Reducing
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Hex Keys, 5064-8211 Screwdrivers De
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Capillary and Megabore ferrule tool
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FID cleaning kit, 9301-0985 GC Budd
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Ink cartridge, 5181-1220 Integrator
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HPIB and GPIB Cables Integrators, I
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Sample Preparation Improve the qual
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Move more quickly toward the method
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R 1 Sulfonic acid-modified divinyl
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O Si O Si (CH2) 17CH3 Si O Si(CH 3)
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Structure of ethyl (C2) silane, cov
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Si O Si O Si OH Structure of diol s
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Additional Agilent SampliQ SPE Phas
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SampliQ Carbon SampliQ Carbon (grap
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Agilent SampliQ Bulk Sorbents www.a
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20-Port Vacuum Manifold Assembly, 5
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Vacuum Manifolds for 96 Well Plates
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Why Filter Your Samples for HPLC?
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Membrane Econofilters, 5185-5830 Re
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Tips & Tools For more information,
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GC and GC/MS For over 40 years, Agi
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GC and GC/MS Maintenance Schedule w
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BTO septa, 5183-4757 Summary of Pre
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Septa Troubleshooting General Purpo
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MS certified split liner, 5188-6576
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Agilent splitless liners Single tap
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Certified gold inlet seal, 5188-536
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Ferrule Selection Recommendations w
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Universal column nut, 5181-8830 Col
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Glass press-fit connections Quartz
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Capillary Flow Technology Supplies
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A B C Valve Supplies Description Pa
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Markes Thermal Desorption Markes Th
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Bar code reader labels Automatic Li
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G1888A Headspace unit G1888A Networ
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Flip Top Inlet Sealing System insta
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Split/Splitless Inlet assembly Spli
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1a 5 6 1b 2 3 1c 1d Cool On-Column
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Tips & Tools For new PTV technology
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Flame Ionization Detector (FID) FID
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FID cleaning kit, 9301-0985 Flame I
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Detector fitting Front ferrule Back
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www.agilent.com/chem/supplies Flame
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www.agilent.com/chem/supplies Nitro
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13 14 17 18 1 3 4 5 6 15 16 8 7 Nit
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Sulfur Chemiluminescence Detector (
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Cool On-Column Inlets for Manual In
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7 5890 FID 2 3 4 5 8 9 6 Flame Ioni
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ECD make up gas adapter ECD packed
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GC Standards GC Qualitative Standar
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GC/MS Systems • Less downtime for
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MS interface column nut, 05988-2006
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Electron Impact (EI) Ion Source Ion
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Tips & Tools Visual appearance is n
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EI high temperature filament, G2590
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Foreline Pump Vacuum Systems and Pu
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MS Test and Performance Samples MS
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Always Quality. Always Innovative.
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Tips & Tools See where quality begi
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Agilent's Über One Test Probe Mixt
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High Efficiency Capillary GC Column
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Spearmint Oil Carrier: A: Helium 25
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Structure of Dimethylpolysiloxane D
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CH 3 CH 3 CH 3 O Si Si O Si CH3 CH3
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CH 3 O Si CH 3 Structure of Dipheny
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CH 3 DB-35ms Chromatograms Environm
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DuraGuard www.agilent.com/chem/supp
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Structure of Dimethylpolysiloxane D
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DB-1 www.agilent.com/chem/supplies
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HP-1 0.32 15 0.25 -60 to 325/350 19
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DB-5 www.agilent.com/chem/supplies
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HP-5 www.agilent.com/chem/supplies
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CH 3 O Si O Si Structure of Dipheny
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O CH 3 C 6H 5 Si O Si CH 3 50% C 6H
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O Si Structure of trifluoropropylme
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DB-1301 • (6%-Cyanopropyl-phenyl)
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-HO H H C C O H H Structure of Poly
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DB-WAX and DB-WaxFF www.agilent.com
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HP-INNOWax Chromatograms Food, Flav
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HP-FFAP Chromatograms Food, Flavors
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Chiral Columns Special Application
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Cyclodex-B Chromatograms Food, Flav
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DB-5ht Chromatograms Food, Flavors
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DB-EVDX Chromatograms Life Sciences
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DB-608 Chromatograms Environmental
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DB-HT SimDis Chromatograms Petroleu
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DB-Dioxin Chromatograms Environment
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DB-VRX Chromatograms Environmental
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HP-VOC • Selectivity engineered f
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PLOT Columns www.agilent.com/chem/s
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GS-Alumina KCl Chromatograms Petrol
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GS-GasPro Chromatograms Environment
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HP-PLOT Q Chromatograms Environment
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Metal Columns DB-ProSteel • Excel
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CAM Chromatograms Industrial Chemic
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Custom GC Column Ordering Even thou
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DB-1, 5 in. cage ID (mm) Length (m)
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DB-17, 5 in. cage ID (mm) Length (m
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DB-1701P, 5 in. cage ID (mm) Length
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GS-Q, 5 in. cage ID (mm) Length (m)
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HP-20M, 5 in. cage ID (mm) Length (
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HP-PLOT Molesieve, 5 in. cage www.a
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Agilent LTM System CAM LTM Column M
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0.20 12 0.33 -60 to 325/350 128-101
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DB-1ms LTM Column Modules ID (mm) L
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DB-17ms LTM Column Modules ID (mm)
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DB-23 LTM Column Modules ID (mm) Le
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DB-5 LTM Column Modules ID (mm) Len
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DB-5.625 LTM Column Modules www.agi
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DB-WAX LTM Column Modules www.agile
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GS-Q LTM Column Modules ID (mm) Len
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HP-5 LTM Column Modules www.agilent
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HP-PLOT Al2O3 "M" LTM Column Module
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HP-PLOT Q LTM Column Modules www.ag
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Fused Silica Tubing Deactivated Tub
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GC Column Application and Method Gu
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GC Columns Stationary Phase Applica
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GC Columns Stationary Phase Applica
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Environmental/EPA Methods Analyte T
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Environmental/EPA Methods EPA Metho
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ASTM Methods Method Designation Met
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Baseline Instability or Disturbance
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Loss of Resolution GC Column Troubl
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www.agilent.com/chem/supplies Solve
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Alphabetical Order By Analyte (Cont
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Environmental Applications, Hydroca
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Environmental Applications, Pestici
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Organochlorine Pesticides I EPA Met
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Chlorinated Pesticides, EPA Method
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Organochlorine Pesticides III Colum
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Nitrogen/Phosphorus Containing Pest
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Herbicides II Column: DB-210 122-02
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Environmental Applications, Semivol
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Semivolatile Compounds, EPA Method
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PBDEs by ECD Column: DB-XLB 15 m x
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PCBs by EPA Method 8082 Column: DB-
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Organotin Compounds II Column: HP-5
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EPA Method 8061 (Phthalate Esters)
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Phenols Column: DB-5ms 122-5532 30
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Environmental Applications, Volatil
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Extended Analyte List for EPA Metho
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EPA Method 551 Column: DB-1 122-103
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EPA Air Analysis Method TO-15 (1 pp
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C1 and C2 Halocarbons (Freons) Colu
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Foods, Flavors and Fragrance Applic
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Fragrance Reference Standard II Col
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Flavor Mixture Column: ULTRA 2 1909
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Peppermint Oil Column: DB-WAX 122-7
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Rosemary Oil Column: Cyclosil-b 112
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Strawberry Syrup Column: HP-INNOWax
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Organic Acids Column: DB-FFAP 122-3
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Polyunsaturated Fatty Acid Methyl E
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FAME Standard II Column: DB-225 127
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Butter Triglycerides I Column: DB-5
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Alcohols II Column: DB-WAXetr 123-7
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Free Organic Acids/C4-C5 Isomers Co
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Amines and Nitriles Column: DB-5ms
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Aldehydes and Ketones Column: DB-1
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PFBHA Derivative Column: DB-1 123-1
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Impurities in Styrene Column: DB-WA
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Esters I Column: DB-1 125-1034 30 m
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Glycols I Column: DB-WAX 124-7032 3
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Ethylene Glycol Mixture Column: ULT
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Halogenated Hydrocarbons II Column:
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Solvents II Column: DB-WAXetr 123-7
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Common Industrial Solvents Column:
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Acrylate Impurities II Column: DB-1
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Phenols I Column: HP-5ms 19091S-433
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Ethylene Oxide Column: DB-WAX 122-7
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Life Science Applications Benzodiaz
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Drug Screen Column: DB-1ms 122-0132
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Anticonvulsants Column: DB-1 125-10
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Barbiturates Column: DB-35ms 122-38
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Hallucinogens Column: DB-17ms 122-4
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Over-the-Counter Pain Killers - TMS
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Blood Alcohols I (Static Headspace/
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Blood Pollutants II Column: DB-ALC1
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Residual Solvents Column: DB-624 12
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Selected Oxygenates Column: GS-OxyP
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Natural Gas Column: HP-PLOT Al2O3 S
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Propylene Column: GS-Alumina 115-35
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Extended Hydrocarbon Analysis I Col
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Refinery Gas I Column: HP-PLOT Q 19
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Sulfur Gas Analysis in Light Hydroc
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Sulfur Compounds in Propylene (1 pp
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Aromatics Analysis - Ethylbenzene I
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Denatured Fuel Ethanol-ASTM D5501 C
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Simulated Distillation Column: DB-2
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n-Paraffin Standard Column: DB-HT S
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LC and LC/MS Run samples up to 20x
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Universal Connection Capillaries
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Plastic tubing cutter, 8710-1930 PE
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Stainless steel fittings, 5062-2418
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Semi prep filter, 5064-8273 High pr
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Glass solvent filter degasser, 3150
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www.agilent.com/chem/supplies Manua
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Stainless steel sample loops www.ag
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Syringes for manual injection valve
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1100/1200 Series LC Maintenance Sch
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Mounting tool, 0100-1710 Capillary
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Pumps www.agilent.com/chem/supplies
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Outlet ball valve, G1311-60012 Acti
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Pump start up kit, G1311-68710 Solv
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Prep filter, 5065-4500 Prep bottle,
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1100/1200 Series LC System Needles
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Sample loop, 01078-87302 www.agilen
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G1367A well plate autosampler G1387
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G2255-68700 Vial plate, 5022-6539 W
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G2260A 1100/1200 Series Preparative
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1100/1200 Series Fraction Collector
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1200 Series LC Solution Kits Biocom
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1100/1200 Series LC System Connecti
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1200 Rapid Resolution Connection Ca
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Standard flow cell Standard flow ce
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Micro flow cell High pressure flow
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8 Deuterium longlife lamp, 2140-081
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Diode Array Detector (DAD)/Multiple
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Diode Array Detector (DAD)/Multiple
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Stainless steel front ferrules, 518
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Piston seals, 5063-6589 PTFE frits,
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Deuterium lamp, G1314-60100 Variabl
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6100 Series Single Quadropole LC/MS
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6200 Series TOF LC/MS Supplies www.
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6300 Series Ion Trap LC/MS Supplies
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6400 Series Triple Quadropole LC/MS
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6500 Series Accurate-Mass Q-TOF LC/
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Quiet Cover Quiet Cover Agilent has
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Column and Mobile Phase Guidelines:
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pH and Mobile Phase The choice of m
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Analyte charge Silica surface charg
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Method development at mid pH (4-9)
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USP Designations The US Pharmacopei
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USP Designations USP Packing Materi
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USP Designations USP Packing Materi
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Cartridge/Guard Cartridge Systems C
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This icon identifies stand-alone gu
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This icon identifies preparative gu
Page 583 and 584: HPLC Column Protection Column Prote
Page 585 and 586: ZORBAX Rx-SIL uniform sub particles
Page 587 and 588: ZORBAX Reversed-Phase Columns The f
Page 589 and 590: Poroshell 120 • High efficiency a
Page 591 and 592: ZORBAX Eclipse Plus: Best Peak Shap
Page 593 and 594: ZORBAX Eclipse Plus Hardware Descri
Page 595 and 596: ZORBAX Eclipse PAH Hardware Descrip
Page 597 and 598: Good Peak Shape Over a Wide pH Rang
Page 599 and 600: Selectivity Changes for Basic Compo
Page 601 and 602: ZORBAX Eclipse XDB Hardware Descrip
Page 603 and 604: ZORBAX Eclipse XDB Hardware Descrip
Page 605 and 606: StableBond SB-C18 Shows Excellent S
Page 607 and 608: Five Different Bonded Phases Provid
Page 609 and 610: ZORBAX 80Å StableBond Hardware Des
Page 611 and 612: ZORBAX 80Å StableBond www.agilent.
Page 613 and 614: Improved Peak Shape of Basic Compou
Page 615 and 616: ZORBAX Bonus-RP Provides Unique Sel
Page 617 and 618: O O Si Si Silica Support C18 C18 No
Page 619 and 620: Extend-C18 Provides Good Peak Shape
Page 621 and 622: ZORBAX 80Å Extend-C18 Hardware Des
Page 623 and 624: ZORBAX Rx Hardware Description Size
Page 625 and 626: LiChrospher Reversed-Phase Cartridg
Page 627 and 628: Asahipak Reversed-Phase Columns Asa
Page 629 and 630: ZORBAX Normal Phase Columns For nor
Page 631 and 632: Normal Phase Columns Based on ZORBA
Page 633: LiChrospher Normal-Phase Cartridge
Page 637 and 638: Poroshell 120 • High efficiency a
Page 639 and 640: ZORBAX Rapid Resolution High Throug
Page 641 and 642: Rapid Resolution HT Provides Double
Page 643 and 644: Long Lifetime of RRHT Columns at El
Page 645 and 646: ZORBAX Rapid Resolution High Throug
Page 647 and 648: Rapid Resolution Columns Reduce Ana
Page 649 and 650: ZORBAX Rapid Resolution 3.5 µm Ecl
Page 651 and 652: ZORBAX Solvent Saver • Provide 60
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Page 655 and 656: Separation of a Tryptic Digest on Z
Page 657 and 658: ZORBAX PrepHT • Easy scale-up fro
Page 659 and 660: ZORBAX PrepHT columns are designed
Page 661 and 662: ZORBAX PrepHT Eclipse XDB (require
Page 663 and 664: Agilent Prep LC Columns • High lo
Page 665 and 666: Agilent Prep LC Columns www.agilent
Page 667 and 668: Separation of Ethiazide (diuretic d
Page 669 and 670: GPC-SEC start-up kit GPC-SEC Start-
Page 671 and 672: Organic Standard Chromatograms Aque
Page 673 and 674: PLgel Columns Agilent Technologies
Page 675 and 676: Polystyrene: Calibration Curves for
Page 677 and 678: Separation of Simple-Sugar and Suga
Page 679 and 680: High Resolution of 24 Amino Acids U
Page 681 and 682: ZORBAX Method Development Kits ZORB
Page 683 and 684: ZORBAX Method Validation Kits ZORBA
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Porous Shell SOLID CORE 5 µm Poros
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MicroBore Poroshell 300 Columns Pro
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Short-Chain ZORBAX 300SB-C3 is Stab
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ZORBAX 300Å StableBond Hardware De
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Novel Bidentate C18-C18 Bonding for
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LC/MS Analysis of Angiotensin on Ex
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ZORBAX GF-250 and GF-450 Gel Filtra
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ZORBAX GF-250 Shows Extended Column
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ZORBAX Bio-SCX Series II Provides M
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High Sensitivity with Capillary Col
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Proteins in a Complex Sample by 2-D
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Multiple Affinity Removal System -
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Column Specifications Multiple Affi
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Multiple Affinity Removal System -
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Tips & Tools The Spin Cartridge Rea
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Luer-Lok syringe, 5188-5250 Luer-Lo
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Protein In-gel Tryptic Digestion Ki
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Peptide Cleanup Pipette Tips Peptid
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HPLC Troubleshooting Symptom Type P
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LC/MS Troubleshooting Symptom Type
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Analgesics: High-Speed PrepHT Isocr
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Cough Formula Mixture: Fast and Eff
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Use ZORBAX Extend-C18 for Alternate
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Peptides: Effect of TFA Concentrati
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Peptide Map - Electronic Flow Contr
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Peptide RP-HPLC/ESI-MS Using NH4OH
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Catecholamines/Biogenic Amines: Rap
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Proteins: Effect of Bonded Phase Co
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BSA Tryptic Digest on RRHT Column:
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Polymethacrylate: GPC Separation Co
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Triton X-114: Decreasing Run-time b
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Organic Acids Separated on ZORBAX S
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Anilines, Separation of Substituted
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Explosives from Soil Extract Column
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Herbicides: Use of Short, Small Par
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Triazine Pesticides on Bonus-RP and
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Plant Hormones: Rapid Gradient Elut
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Aromatic Flavorings Column: Eclipse
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Carbohydrates in Colas Column: ZORB
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Flavoring Agents Column: ZORBAX SB-
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Parabens: High Speed Separation Col
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Water-Soluble Vitamins Column: ZORB
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Fast Vitamin E Analysis on Rapid Re
Page 771 and 772:
Xanthines: Higher Resolution, Same
Page 773 and 774:
Anesthetics, Local: Bonded Phase Se
Page 775 and 776:
Antihistamines Column: ZORBAX Rx-C8
Page 777 and 778:
Chiral Separation of Fluoxetine Ena
Page 779 and 780:
Chiral Separation of Tolperison Ena
Page 781 and 782:
Metronidazole: Updating USP Methods
Page 783 and 784:
Opiate Drugs, Basic Compounds Showi
Page 785 and 786:
Steroids Column A: Eclipse XDB-Phen
Page 787 and 788:
Tricyclic Antidepressants, Screenin
Page 789 and 790:
Dexamethasone, USP Method: Rapid An
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Sulfonamides - Fast Analysis with R
Page 793 and 794:
CE and CE/MS Achieve the sensitivit
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Tips & Tools Agilent offers a varie
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µPAGE Basic Kits Includes 3 µPAGE
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Extended Light Path (Bubble Cell) B
Page 801 and 802:
www.agilent.com/chem/supplies CE an
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www.agilent.com/chem/supplies CE an
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Alignment Interfaces Capillary cass
Page 807 and 808:
CE/MS Accessories www.agilent.com/c
Page 809 and 810:
CE Standards & Reagents Premade buf
Page 811 and 812:
CE System Start-up and Test Kits Ch
Page 813 and 814:
Accessories www.agilent.com/chem/su
Page 815 and 816:
ICP-MS The power you need for ultra
Page 817 and 818:
Gas Line Supplies www.agilent.com/c
Page 819 and 820:
Peristaltic Pump Tubing and Supplie
Page 821 and 822:
Concentric Nebulizer, quartz Use wi
Page 823 and 824:
Quartz spray chamber, G1820-65337 S
Page 825 and 826:
Interface Cone Guide for Agilent IC
Page 827 and 828:
Electron multiplier, 5184-1983 Ion
Page 829 and 830:
Integrated Autosampler Supplies Des
Page 831 and 832:
ISIS Supplies www.agilent.com/chem/
Page 833 and 834:
Miscellaneous Supplies Description
Page 835 and 836:
Single Element Standards Descriptio
Page 837 and 838:
UV-Vis Keeping your system operatin
Page 839 and 840:
Semi-Micro Cell with PTFE Lid Semi-
Page 841 and 842:
Round Aperture Flow Cell Rectangula
Page 843 and 844:
Deuterium lamp assembly, 2140-0605
Page 845 and 846:
UV-Vis Standards & Reagents Our che
Page 847 and 848:
0905-1175..........................
Page 849 and 850:
123-0131LTM........................
Page 851 and 852:
125-5032E..........................
Page 853 and 854:
19091N-133.........................
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200-0185...........................
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5064-8286..........................
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5183-4428..........................
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5982-4411..........................
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840300-908.........................
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959746-906.........................
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G1530-61580........................
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G3270-80026........................
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1,4-Butanediol ....................
Page 873 and 874:
Dicrotophos .......................
Page 875 and 876:
1,2,3,4,6,7,8-Hepta chloro dibenzof
Page 877 and 878:
Octadecane ........................
Page 879 and 880:
Triethylenetetramine (4 isomers) ..
Page 881 and 882:
Caps 8-425 Screw Top No Septa......
Page 883 and 884:
GC Installation Kits Basic Installa
Page 885 and 886:
Moisture Traps Cont. Silica Gel, Gr
Page 887 and 888:
Vials Cont. 2 mL Glass ............
Page 889 and 890:
Easy Ways To Order* • For Interna
Page 891 and 892:
Agilent Technologies Order Form Agi
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