TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Pharmaceutical Applications
Chiral Separation of Hexobarbitol
Column: Chiradex
79925CB-584
4.0 x 250 mm, 5 µm
Mobile Phase: Methanol/water, 20:80
Flow Rate: 1.0 mL/min
Detector: UV 220 nm
Sample: Hexobarbitol
778 www.agilent.com/chem/supplies
12
O
24 36
Retention Time (min)
48
N N
Chiral Separation of S- and R-Norfluoxetine
using Ultron ES-Pepsin
R-Norfluoxetine S-Norfluoxetine
Column: Ultron ES-OVM Chiral
724111653
4.6 x 250 mm, 10 µm
Mobile Phase: 6/94 (v/v) MeOH / 20 mM KH2PO4
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detector: UV 225 nm
Sample: 50 µg/mL of 2:3 mixture R- : S-Norfluoxetine
0 3 6 9 12 15 18 21 24 27 30
Time (min)
Courtesy of D.S. Ristry and V.S. Sharp, Eli Lilly and Co. LCPC019
Chiral Separation of Salbutamol on Ultron ES-Pepsin
Column: Ultron ES-Pepsin
822111631A
4.6 x 150 mm, 5 µm
Mobile Phase: 20 mM phosphate buffer, pH 6.0
Flow Rate: 1.0 mL/min
Temperature: 25° C
Detector: UV 220 nm
Sample: 20 µL containing 0.35 µg Salbutamol Mixture
10
Time (min)
LCPC020
O
LCPC017
O