TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
BioPharmaceutical Applications
Glycosylated proteins: Large Molecules
on Poroshell 300SB-C18 and 300SB-C8
Column A: Poroshell 300SB-C18
661750-902
1.0 x 75 mm, 5 µm
Column B: ZORBAX Poroshell 300SB-C8
661750-906
1.0 x 75 mm, 5 µm
Column C: ZORBAX 300SB-C18
865630-902
1.0 x 50 mm, 3.5 µm
Mobile Phase: a: 0.1% TFA in H20
b: 0.07% TFA in ACN
Flow Rate: a, b: 0.454 mL/min
c: 0.071 mL/min
Gradient: a, b:
0 min 5% B
10 min 100% B
c:
0 min 5% B
50 min 100% B
Temperature: 70°C
Detector: DAD 212nm, 1. 7 µL flow cell,