TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Pharmaceutical Applications
Ibuprofen: Optimizing Selectivity with
RRHT Columns
Column A: SB-C8
827975-906
4.6 x 50 mm, 1.8 µm
Column B: Eclipse XDB-C8
927975-906
4.6 x 50 mm, 1.8 µm
Mobile Phase: A: 63% water
B: 37% acetonitrile + 1.8 mL H3PO4
Flow Rate: 2.0 mL/min
Temperature: Ambient
Detector: UV 254 nm
Sample: Ibuprofen Oral Suspension
Analgesics: High Speed Separation
Column: ZORBAX SB-C18
833975-902
4.6 x 30 mm, 3.5 µm
Mobile Phase: 1 mM octane sulfonic acid, Na salt, pH 2.5,
or 1% formic acid: ACN (80:20)
Flow Rate: 2 mL/min
Temperature: 70°C
Detector: UV 275 nm with standard flow cell (13 µL)
Sample: Analgesics, 1 µL
772 www.agilent.com/chem/supplies
mAU
120
80
40
0
mAU
120
80
40
0
A
0 1
2 3
Time (min)
4 5
B
1
1
2
2
3
3
1. Benzophenone
2. Ibuprofen
0 1
2 3
Time (min)
4 5
4 5
0 0.2 0.4 0.6 0.8 1.0
4
0 0.2 0.4 0.6
Time (min)
0.8 1.0
6
5
7
6
1. Acetaminophen (4-acetamidophenol)
2. Caffeine
3. 2-acetamidophenol
4. Acetanilide
5. Aspirin (acetosalicylic acid)
6. Salicylic acid
7. Phenacetin (acetophenetidin)
7
α = 1.58
Rs = 10.0
α = 1.35
Rs = 6.3
Ion-Pair Mobile Phase
Volatile Mobile Phase
LCPC006
LCPC003