TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
Pharmaceutical Applications
Pharmaceutical Applications
Separation of Pharmaceutical Cardiac Drugs
on Eclipse Plus C18
Column: Eclipse Plus C18
959996-902
4.6 x 100 mm, 5 µm
Mobile Phase: A: 20 mM Ammonium Acetate, pH 4.8:
B: ACN
Flow Rate: 1 mL/min
Gradient: 10 – 90% in 10 minutes
Detector: UV 254 nm
Fast and Ultra-Fast Analysis of Basic Compounds
on Eclipse Plus
Column: Eclipse Plus C18
959941-902
4.6 x 50 mm, 1.8 µm
Mobile Phase: A: 50% 8 mM K2HPO4, pH 7
B: 50% ACN
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detector: UV 254 nm
Publication: 5989-4934EN
Sample: Berberine, 0.4 mg/mL, 2 µL
1
0 2 4 6 8
Time (min)
A
Tf = 1.00
1
O
O N+ Cl-
770 www.agilent.com/chem/supplies
2
3
4
1. Berberine
5
6
7
C18Cardiac
0 0.5 1.0
1.5
Time (min)
2.0
2.5 3.0
OCH 3
OCH 3
1. Procainamide
2. Furosemide
3. Eletriptan
4. Buspirone
5. Diltiazen
6. Dipyridamole
7. Pioglitazone
LCEC002