TABLE OF CONTENTS - BGB Analytik AG

Environmental Applications, Pesticides and Herbicides
CLP Pesticide Analysis
Column: DB-XLB
121-1222
20 m x 0.18 mm, 0.18 µm
Carrier: H2, constant flow, 77.3 cm/s at 120 °C
Oven: 120°C for 0.49 min
120°C to 160°C at 59.4°/min
160°C to 260°C at 23.7°/min
260°C to 300°C (1.69min) at 35.6°/min
Injection: Pulsed Splitless, 220°C
Pulse pressure & time: 35psi for 0.5 min Flow
ramp at 6.25 min of 99 mL/min 2 to 3 mL/min
2 mm ID liner
Detector: µ-ECD, 320°C
Ar/CH4 (P5) makeup gas at 60mL/min
Sample: 0.5 µL, 50 ppb
Column: DB-XLB
123-1232
30 m x 0.32 mm, 0.25 µm
Carrier: He, constant flow, 38 cm/s at 120ºC
Oven: 120ºC for 1.17 min
120ºC to 160ºC at 25º/min
160ºC to 260ºC at 10º/min
260ºC to 300ºC (4 min) at 15º/min
Injection: Pulsed Splitless, 220ºC
Pulse pressure & time: 35 psi for 1.15 min
Detector: µ-ECD, 320ºC
Ar/CH4 (P5) makeup gas at 60 mL/min
Sample: 2 µL, 50 ppb
Environmental Applications, Pesticides and Herbicides
Faster Method (using a High Efficiency GC Column and H2 carrier)
3.6e + 07
3.4e + 07
3.2e + 07
3e + 07
2.8e + 07
2.6e + 07
2.4e + 07
2.2e + 07
2e + 07
1.8e + 07
1.6e + 07
1.4e + 07
1.2e + 07
1e + 07
8000000
6000000
4000000
2000000
1. TCMX
2. Alpha BHC
3. Gamma BHC
4. Beta BHC
5. Delta BHC
6. Heptachlor
7. Aldrin
8. Heptachlor Epoxide
9. Gamma Chlordane
10. Alpha Chlordane
11. Endosulfan I
Original Method (using a 0.32 mm ID column and Helium carrier)
1.9e + 08
1.8e + 08
1.7e + 08
1.6e + 08
1.5e + 08
1.4e + 08
1.3e + 08
1.2e + 08
1.1e + 08
1e + 08
9e + 07
8e + 07
7e + 07
6e + 07
5e + 07
4e + 07
3e + 07
2e + 07
1e + 07
0.50
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1
2
3
4
Time (min)
5, 6
7
8
9 10
12
13
11
14 15
1618
1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00
1
2
3
4
5, 6
7
Time (min)
8
1
12. 4,4' DDE
13. Dieldrin
14. Endrin
15. 4,4' DDD
16. Endosulfan II
17. 4,4' DDT
18. Endrin Aldehyde
19. Endosulfan Sulfate
20. Methoxychlor
21. Endrin Ketone
22. DCB
9 10
12
11 13
14 15
18
16 19
2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00
Contract Laboratory Program (CLP) pesticide analysis on High Efficiency (0.18 mm I.D.) GC columns. In this example, the analysis of 22 CLP pesticides were achieved in 16
minutes using the original method, whereas the improved method was completed in just under 7 minutes. That's a 56% faster sample run time.
17
20
21
17
19
20
21
22
22
GCHE006
GCHE007
GC and GC/MS
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