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TABLE OF CONTENTS - BGB Analytik AG

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LC and LC/MS<br />

ZORBAX Capillary and Nano<br />

2D LC/MS Analyses Using ZORBAX Capillary and Nano LC Columns<br />

Typical Column Configuration for 2D HPLC<br />

1100/1200 Series micro<br />

well plate autosampler<br />

SCX Strong cation<br />

exchange column<br />

1100/1200 Series<br />

quaternary pump<br />

Flow path of the Agilent 1100/1200 Series Nanoflow Proteomics Solution system.<br />

1. Sample loading, elution from SCX and trapping on enrichment column<br />

2. Valve switch in column compartment, elution from enrichment column;<br />

separation on RP, and MS anaylsis<br />

704 www.agilent.com/chem/supplies<br />

1.<br />

2.<br />

1100/1200 Series micro<br />

well plate autosampler<br />

SCX Strong cation<br />

exchange column<br />

1100/1200 Series<br />

quaternary pump<br />

Waste<br />

Waste<br />

Reversed-phase<br />

enrichment column<br />

Waste<br />

Reversed-phase column<br />

1100/1200 Series<br />

column compartment<br />

Nanopump<br />

Detection<br />

(1100/1200 Series<br />

LC/MSD Trap)<br />

Reversed-phase<br />

enrichment column<br />

Waste<br />

Reversed-phase column<br />

1100/1200 Series<br />

column compartment<br />

Nanopump<br />

Detection<br />

(1100/1200 Series<br />

LC/MSD Trap)

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