TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
ZORBAX 300Å StableBond
Capillary Columns for HPLC Analyses
with UV and MS Detection
Column: ZORBAX 300SB-C18
5064-8263
0.3 x 150 mm, 5 µm
Mobile Phase: 5-55% B in 50 min, to 85% B
from 55-57 min
A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in ACN
Flow Rate: 5.5 µL/min
Detector: 206 nm
MS Conditions: LC/MS: Pos. Ion ESI with
LC/MSD trap-Vcap 4000 V
Drying Gas Flow: 7 L/min
Drying Gas Temperature: 250°C
Nebulizer: 15 psi
Capillary Exit Volt: 50 V
Max Accum Time: 300 ms
Total Averages: 3
Isolation Width: 3 m/z
Frag Ampitude: 1.0 V
Sample: 100 nL
Beta Casein Digest (4 pmol)
A ZORBAX 300SB-C18 capillary column (0.3 mm ID) is used for the
separation of the protein digest. Detection is by both UV and Electrospray
MS. MS detection can be used for identification of peptide fragments.
ZORBAX Nano Columns For High Sensitivity
Protein Digest Analysis by LC/MS
Column: ZORBAX 300SB-C18
5065-9911
0.075 x 150 mm, 3.5 µm
Mobile Phase: A: Water + 0.1% Formic Acid,
B: ACN + 0.1% Formic Acid
Flow Rate: 600 nl/min
Gradient: 2% B to 52% B in 25 min
Detector: Positive Ion Nano
Electrospray MS
Sample: 100 fm (1 µl) Digest
of 8 Proteins
A ZORBAX nano HPLC column, 0.075 mm ID, is used for high
sensitivity LC/MS analysis of a protein digest sample.
Intens.
690 www.agilent.com/chem/supplies
1.5
1.0
0.5
UV at 206 nm
% Relative Abundance
100
80
60
40
20
0
x10
0.8
0.6
0.4
0.2
0.0
UV
MS
10
10
15
15
20 25 30 35 40 45 50
Time (min)
20 25 30 35 40 45 50
Time (min)
2.5 5.0 7.5 10.0 12.5
Time (min)
15.0 17.5 20.0 22.5
LCSB007
LCSB008