TABLE OF CONTENTS - BGB Analytik AG

Environmental Applications, Semivolatiles
US EPA Method 8270 Short Mix
Column: DB-5ms Ultra Inert
122-5532UI
30 m x 0.25 mm, 0.25 µm
Carrier: Helium constant flow 30 cm/s
Oven: 40°C (1 min) to 100°C (15°C/min),
10°C to 210°C (1 min),
5°C/min. to 310°C (8 min)
Injection: Split/splitless; 260°C, 53.7 mL/min.
total flow, purge flow 50 mL/min. on
at 0.5 min., gas saver flow 80 mL/min.
on at 3.0 min.
Detector: MSD source at 300°C, quadrapole
at 180°C, transfer line at 290°C,
full scan m/z 50-550
Sample: 1.0 µL splitless injection, 5 ng each
component on column
Suggested Supplies
Liner: Direct connect, dual taper, deactivated,
4 mm ID, G1544-80700
Syringe: 10 µl tapered, FN 23-26s/42/HP,
5181-1267
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www.agilent.com/chem/supplies
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Environmental Applications, Semivolatiles
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5.00 10.00 15.00 20.00 25.00 30.00
Time (min)
18
EPA8270
1. N-nitrosodimethylamine
2. Aniline
3. 1,4 dichoronezene-D4
4. 1,4 dichlorobenzene
5. Naphthalene-D8
6. Acenapthene-D10
7. 2,4-dinitrophenol
8. 4-nitrophenol
9. 2-methyl-4,6-dinitrophenol
10. Pentachlorophenol
11. 4-aminobiphenyl
12. Phenanthrene- D10
13. Benzidine
14. Chrysene-D12
15. 3,3'-dichlorobenzidine
16. Benzo [b] fluoranthene
17. Benzo [k] fluoranthene
18. Perylene-D12
Semivolatile analysis using methods similar to US EPA Method 8270 is becoming increasingly important in environmental laboratories worldwide. Acidic compounds
such as benzoic acid or 2,4-dinitrophenol – along with strong bases such as pyridine or benzidine – are examples of active species found in the semivolatile sample set.
This DB-5ms Ultra Inert column demonstrates excellent inertness performance for these difficult analytes.
Analysis of Semivolatiles
Column A: DB-5.625
122-5632
30 m x 0.25 mm, 0.50 µm
Column B: DB-5.625
121-5622
20 m x 0.18 mm, 0.36 µm
Carrier: He constant-flow mode 1.1 mL/min
Oven: 40°C (1 min), 25°C/min to 320°C
4.80 min hold
Injection: Splitless 0.5 µL injected at 300°C, QuickSwap
pressure 5.0 psi during acquisition, 80.0 psi during
backflush with inlet set to 1.0 psi during backflush
Detector: Agilent 5975C Performance Turbo MSD equipped
with 6 mm large-aperture drawout lens,
part number G2589-20045
Translating 0.25 mm ID column method to 0.18 mm ID format
results in 32% reduction in analysis time. Resolution of
77 peaks of interest is also maintained for the faster
0.18 mm ID separation.
A
4.00 8.00
12.00
16.00
20.00
24.00
Time (min) GCHE001
U.S. EPA Method 8270, 5 ng/mL System Performance Check Compounds Chromatogram
using a DB-5.625, 30 m x 0.25 mm x 0.5 µm
B
4.00 6.00 8.00
10.00 12.00
Time (min)
14.00 16.00
U.S. EPA Method 8270, 5 ng/mL System Performance Check Compounds Chromatogram
using a DB-5.625, 20 m x 0.18 mm x 0.36 µm
GCHE002
GC and GC/MS
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